Detection of multiple strains of Pasteurella multocida in fowl cholera outbreaks by polymerase chain reaction-based typing

Avian Pathology : Journal of the W.V.P.A
S B ShivachandraS K Srivastava

Abstract

Applicability of molecular methods for the detection and differentiation of Pasteurella multocida strains involved in two separate fowl cholera outbreaks in a single poultry farm was investigated. A total of 12 and 18 strains of P. multocida obtained from two separate outbreaks were subjected to phenotypic and genotypic characterization. Phenotypically, all strains were similar; however, DNA-based techniques by employing polymerase chain reaction (PCR) assays were found to be highly specific and sensitive for rapid detection and differentiation of strains. All 30 strains gave amplicons of approximately 460 bp and approximately 1,044 bp specific for P. multocida and capsular serogroup A in the Multiplex Capsular PCR typing system. Molecular typing techniques such as repetitive extragenic palindromic PCR, enterobacterial repetitive intergenic consensus PCR and single primer PCR differentiated all 30 strains into different profiles. However, similar patterns of genome fragments were observed among all strains following restriction endonuclease analysis using the enzyme HpaII. The current investigation revealed involvement of the same and multiple strains of P. multocida in two outbreaks. The results also indicated that molecular m...Continue Reading

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Citations

Feb 13, 2007·Veterinary Research Communications·S B ShivachandraP Chaudhuri
Apr 23, 2011·Veterinary Microbiology·E J HotchkissR N Zadoks
Aug 7, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yanhong WangXiufan Liu
Jun 1, 2007·Comparative Immunology, Microbiology and Infectious Diseases·S B ShivachandraP Chaudhuri
Nov 15, 2013·Avian Pathology : Journal of the W.V.P.A·Reema SinghConny Turni
Nov 11, 2009·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Anina B J StahelBozena M Korczak
Dec 25, 2007·Nature Reviews. Microbiology·Rotem SorekPhilip Hugenholtz
Jan 1, 2013·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Mads F BertelsenHenrik Christensen
Apr 28, 2021·Scientific Reports·Rine Christopher ReubenIqbal Kabir Jahid

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Methods Mentioned

BETA
PCR
electrophoresis

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