PMID: 9642774Jun 27, 1998Paper

Detection of multiple toxic agents using a planar array immunosensor

Biosensors & Bioelectronics
R R WadkinsF S Ligler

Abstract

A planar array immunosensor, equipped with a charge-coupled device (CCD) as a detector, was used to simultaneously detect 3 toxic analytes. Wells approximately 2 mm in diameter were formed on glass slides using a photoactivated optical adhesive. Antibodies against staphylococcal enterotoxin B (SEB), ricin, and Yersinia pestis were covalently attached to the bottoms of the circular wells to form the sensing surface. Rectangular wells containing chicken immunoglobulin were used as alignment markers and to generate control signals. After removing the optical adhesive, the slides were mounted over a scientific grade CCD operating at ambient temperature in inverted (multipin phasing) mode. A two-dimensional graded index of refraction lens array was used to focus the sensing surface onto the CCD. Solutions of toxins were then placed on the slide. After rinsing, Cy5-labeled antibodies were introduced. The identity and amount of toxin bound at each location on the slide were determined by quantitative image analysis. Concentrations as low as 25 ng/mL of ricin, 15 ng/mL of pestis F1 antigen, and 5 ng/mL of SEB could be routinely measured.

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Citations

Jun 10, 2000·Analytical Biochemistry·C A Rowe-TaittF S Ligler
Jun 30, 2006·Analytical and Bioanalytical Chemistry·Sara Rodriguez-MozazDamià Barceló
Nov 9, 2010·Analytical and Bioanalytical Chemistry·Elizabeth M MillerAaron R Wheeler
Aug 10, 2013·Biosensors & Bioelectronics·Joshua BalsamAvraham Rasooly
Sep 17, 2013·Sensors and Actuators. B, Chemical·Joshua BalsamAvraham Rasooly
Nov 24, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·P B LuppaD W Chan
Jan 10, 2003·Cancer Detection and Prevention·Minoo D F AskariTuan Vo-Dinh
Feb 24, 2001·Biosensors & Bioelectronics·C A Rowe-TaittF S Ligler
Apr 18, 2002·Biosensors & Bioelectronics·Zvi LironFrances S Ligler
Aug 23, 2002·Biosensors & Bioelectronics·Joel P Golden, Frances S Ligler
Nov 13, 2003·Biosensors & Bioelectronics·Darcy J LichlyterOrhan Soykan
Aug 17, 2000·Biosensors & Bioelectronics·C A Rowe-TaittF S Ligler
Jun 6, 2009·Analytical Chemistry·Jason S KimFrances S Ligler
Dec 1, 1999·Environmental Science & Technology·K R Rogers, C L Gerlach
Mar 27, 2001·Nature Reviews. Genetics·L Liotta, E Petricoin
Jan 9, 2004·Applied and Environmental Microbiology·Chris Rowe TaittFrances S Ligler
Dec 8, 2014·Biotechnology Advances·William P BozzaBaolin Zhang
Apr 4, 2006·Journal of Immunological Methods·J W ChungJ C Pyun
Dec 18, 2003·Journal of Applied Microbiology·K E SapsfordF S Ligler
Mar 6, 2003·IEEE Transactions on Bio-medical Engineering·Juntao XuRobert L Pastel
Jul 7, 2007·Drug and Chemical Toxicology·Miroslav PohankaKamil Kuca
Jul 9, 2013·Journal of Microbiological Methods·Xiaoyun YangAleksandr Simonian
Oct 13, 2004·Journal of Applied Toxicology : JAT·Sarah B Tencza, Michael A Sipe
Oct 26, 2006·Analytical and Bioanalytical Chemistry·E MaurizL M Lechuga
Aug 16, 2005·Clinical Chemistry·Catherine J ChaseDavid A Kulesh
Dec 13, 2000·Journal of Molecular Recognition : JMR·E R GoldmanG P Anderson
Feb 24, 2001·Journal of Food Protection·A Rasooly
Jun 16, 2005·Journal of Food Protection·Shashi K Sharma, Richard C Whiting
Oct 24, 2003·Journal of Food Protection·Lisa C Shriver-LakeFrances S Ligler
Dec 17, 2008·Analytical Chemistry·Arghavan ShabaniRosemonde Mandeville
Apr 26, 2003·Analytical Chemistry·Mary T McBrideKodumudi S Venkateswaran
Jan 31, 2002·Analytical Chemistry·K E SapsfordF S Ligler
Apr 28, 2005·Biosensors & Bioelectronics·Chris Rowe TaittFrances S Ligler
Apr 30, 2005·Analytical Chemistry·Rory StineCara-Lynne Schengrund
Dec 30, 2004·Analytical Chemistry·Mohammed ZourobNicholas J Goddard
Aug 21, 2001·Analytical Chemistry·J M DodsonF S Ligler
Oct 12, 1999·Analytical Chemistry·T E PlowmanW M Reichert
Feb 13, 2002·Analytical Chemistry·Frances S LiglerOmowunmi A Sadik
Feb 9, 1999·Analytical Chemistry·C A RoweF S Ligler
Nov 16, 2006·Analytical Chemistry·Qian MeiZ Hugh Fan

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