PMID: 9537032Jan 1, 1980Paper

Detection of muramidase (lysozyme)-secreting leucocytes at the single-cell level by a protein A plaque assay

Scandinavian Journal of Immunology
C I Smith, L Hammarström

Abstract

Using a modification of the protein A plaque assay, muramidase (lysozyme)-producing leucocytes were detected as plaque-forming cells. In the presence of anti-muramidase Ig and complement the secreted lysozyme resulted in lysis of protein-A-coated target erythrocytes. By the use of a monolayer technique individual plaque-forming cells could be identified by staining procedures. Granulocytes as well as monocytes were found to produce muramidase and thus to form plaques. This method could serve as a useful tool when studying lysozyme secretion. Furthermore, by the use of appropriate antisera, this method could be employed for the study of any cell type (any secretion), provided enough molecules are being secreted.

References

Feb 21, 1977·Biochemical and Biophysical Research Communications·J M Fernández-Sousa, R Rodriguez
Jul 1, 1979·European Journal of Immunology·C I Smith, L Hammarström
May 1, 1978·The Biochemical Journal·M Z Atassi, C L Lee
Aug 1, 1976·European Journal of Immunology·E GronowiczF Melchers
Apr 5, 1977·Biochemistry·T J WhiteA C Wilson
Jun 1, 1976·Scandinavian Journal of Immunology·A Bøyum
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Jan 1, 1980·Scandinavian Journal of Immunology·A Freijd, T Kunori

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Citations

Jan 1, 1985·Medical Microbiology and Immunology·L Hammarström, C I Smith

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