Detection of murine typhus infection in fleas by using the polymerase chain reaction.

Journal of Clinical Microbiology
L WebbA F Azad

Abstract

Polymerase chain reaction (PCR) amplification of DNA was used to detect the etiologic agent of murine typhus, Rickettsia typhi, in experimentally infected adult fleas. A primer pair derived from the 17-kilodalton antigen sequence of typhus and spotted fever group rickettsiae was used to amplify a 434-base-pair (bp) fragment of the genome of the murine typhus rickettsiae. The amplified 17-kilodalton protein antigen-specific sequence was detected in ethidium bromide-stained agarose gels in individual fleas as early as 2 days after exposure to rickettsemic rats (two of six tested). The 434-bp sequence was not detected in uninfected control fleas. A dot hybridization assay used to detect the 434-bp fragment was also specific and about 100-fold more sensitive than the agarose gel PCR assay. Since the PCR assay employed a boiled extract of triturated fleas, both PCR and an antigen capture enzyme-linked immunosorbent assay (ELISA) could be performed on the same individual flea homogenate. The ELISA identified 12 infected fleas out of 29 randomly selected fleas, compared with 14 specimens which were positive by PCR. The PCR assay detected rickettsiae in samples in which no viable rickettsiae were detected by plaque assay. Like the ELIS...Continue Reading

References

Jan 1, 1990·Annual Review of Entomology·A F Azad
May 1, 1989·The American Journal of Tropical Medicine and Hygiene·M E DobsonJ G Olson
Feb 15, 1989·Analytical Biochemistry·G H KellerM M Manak
Sep 1, 1989·Journal of Bacteriology·B E Anderson, T Tzianabos
Jan 1, 1988·The Journal of Experimental Medicine·D K ShibataW J Martin
Jun 1, 1987·Journal of Bacteriology·B E AndersonW J Bellini
May 1, 1985·The American Journal of Tropical Medicine and Hygiene·A Farhang Azad, R Traub
Sep 1, 1984·The American Journal of Tropical Medicine and Hygiene·S Arango-JaramilloC L Wisseman
Jan 1, 1982·Annual Review of Microbiology·E Weiss

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Citations

Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·A K BejR M Atlas
Jan 1, 1993·Microbiology and Immunology·T Uchida
Jan 14, 2004·Emerging Infectious Diseases·Márcio Antonio Moreira GalvâoDavid H Walker
Aug 4, 2010·Emerging Infectious Diseases·Karla A OliveiraDonald H Bouyer
Jun 25, 2013·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·K NilssonC Påhlson
Jul 31, 2014·Emerging Infectious Diseases·Jorge E Zavala-CastroJorge E Zavala-Velázquez
Feb 20, 2015·Emerging Infectious Diseases·Lucas S BlantonDavid H Walker
Jun 6, 2019·Experimental & Applied Acarology·Sebastián Muñoz-LealMarcelo B Labruna
Feb 17, 2006·Annals of the New York Academy of Sciences·Aaron Medina-SanchezDavid H Walker
Apr 1, 2005·Journal of Medical Entomology·Heather L StevensonDavid H Walker
Nov 1, 2008·Vector Borne and Zoonotic Diseases·Mark P NelderWilliam Wills
Oct 25, 2008·Revista Do Instituto De Medicina Tropical De São Paulo·Manoella Campostrini Barreto ViannaSolange Maria Gennari
Jan 25, 2008·Emerging Infectious Diseases·Nathan B UnsworthStephen R Graves
Mar 6, 2010·Emerging Infectious Diseases·Phillip C WilliamsonSamuel F Atkinson
May 21, 2015·Microbiology·Edyta Konecka, Ziemowit Olszanowski
Nov 28, 2015·Journal of Vector Ecology : Journal of the Society for Vector Ecology·Gaspar Peniche-LaraKarla Dzul-Rosado
Jun 9, 2016·The American Journal of Tropical Medicine and Hygiene·Lucas S BlantonDavid H Walker
Dec 31, 2014·The Journal of Parasitology·Will K ReevesRobert R Paul
Jan 21, 2016·Vector Borne and Zoonotic Diseases·Álvaro A Faccini-MartínezMarylin Hidalgo
Aug 17, 2016·Tropical Medicine and Infectious Disease·Edward RabyStephen R Graves
Jan 1, 1990·Annals of the New York Academy of Sciences·A F AzadG A Dasch
Jan 1, 1995·Journal of Clinical Laboratory Analysis·Z Ronai, M Yakubovskaya
May 1, 1991·European Journal of Epidemiology·H H Winkler
Jun 10, 2009·Infection and Immunity·Lonnie O DriskellDavid O Wood
Feb 26, 2009·Emerging Infectious Diseases·Tomasz ChmielewskiStanislawa Tylewska-Wierzbanowska
Oct 19, 2005·Emerging Infectious Diseases·Ju JiangAllen L Richards
Jan 26, 2008·Emerging Infectious Diseases·William J H McBrideDrew Wenck
Jul 24, 2013·The American Journal of Tropical Medicine and Hygiene·Alejandro Ramírez-HernándezMarylin Hidalgo
Nov 19, 2010·Vector Borne and Zoonotic Diseases·Edyta PodsiadłyStanisława Tylewska-Wierzbanowska
Apr 14, 2015·Future Microbiology·Alison Luce-FedrowAllen L Richards
Jun 8, 2007·Emerging Infectious Diseases·Nathan B UnsworthMichael D Nissen
Apr 4, 2006·Cadernos de saúde pública·Luciane Daniele CardosoMárcio Antônio Moreira Galvão
May 14, 2014·Journal of Vector Ecology : Journal of the Society for Vector Ecology·Theeraphap ChareonviriyaphapWei Mei Ching
Aug 26, 2003·Revista Da Sociedade Brasileira De Medicina Tropical·Márcio Antônio Moreira GalvãoDavid Hughes Walker
Sep 14, 2016·Biomédica : revista del Instituto Nacional de Salud·Daly Martínez-OrtizPablo Manrique-Saide
Oct 8, 2019·Epidemiology and Infection·Matthew T RobinsonStuart D Blacksell

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