Detection of Mycobacterium tuberculosis complex in sputum specimens using a loop-mediated isothermal amplification assay in Korea

Journal of Medical Microbiology
Se Hoon MoonMitsuko Seki

Abstract

Tuberculosis (TB), an infectious disease caused by Mycobacterium tuberculosis complex (MTC), remains one of the leading causes of death in the world. In Korea, the current prevalence of multidrug-resistant TB (MDR-TB) poses a major problem. The most common method for diagnosing TB in developing countries is sputum smear microscopy; however, the sensitivity of this test is relatively low and it usually requires well-trained laboratory staff. Cultures of MTC require up to several weeks in sophisticated facilities, such as Biosafety Level 3. Effective diagnostic techniques are necessary to control TB. In Korea, we evaluated a loop-mediated isothermal amplification (LAMP) assay targeting the hspX gene (TB-hspX-LAMP) of MTC. For clinical evaluation, culture confirmation, smear microscopy and TB-hspX-LAMP were performed on 303 sputum specimens obtained from suspected TB patients in Korea. The sensitivity, specificity, positive predictive value and negative predictive value of TB-hspX-LAMP were 71.1, 98.8, 91.4 and 95.1%, respectively, compared with TB culture, which is the gold standard for diagnosis of TB. In contrast, the comparable values of smear microscopy were 24.4, 98.1, 68.8 and 88.2%, respectively. Therefore, we concluded th...Continue Reading

References

Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
May 28, 2004·Journal of Biochemical and Biophysical Methods·Yasuyoshi MoriTsugunori Notomi
Apr 20, 2005·Clinics in Chest Medicine·Daniel Brodie, Neil W Schluger
Mar 23, 2007·The Lancet Infectious Diseases·Thomas HänscheidNancy G Perlmutter
Feb 12, 2008·Current Protocols in Molecular Biology·K Wilson
Jan 16, 2009·MMWR. Morbidity and Mortality Weekly Report·UNKNOWN Centers for Disease Control and Prevention (CDC)
Apr 28, 2009·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Yasuyoshi Mori, Tsugunori Notomi
Sep 29, 2011·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·S MitaraiT Mori
Feb 14, 2014·Global Public Health·Joshua Amo-Adjei
Jan 6, 2015·The Journal of Microbiology·Tsugunori NotomiHidetoshi Kanda
Jan 13, 2015·Analytical Biochemistry·Shigehiko MiyamotoTakaaki Jikihara

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Citations

Oct 27, 2016·BMC Infectious Diseases·Thilini Piushani KeerthirathneSuneth Sithumini Sooriyapathirana
Nov 21, 2017·American Journal of Reproductive Immunology : AJRI·Kazumasa FuwaSatoshi Hayakawa
Sep 25, 2017·Journal of Clinical Laboratory Analysis·Dandan WuDianxing Sun
Aug 22, 2018·Journal of Clinical Laboratory Analysis·Nuntita SingpanomchaiPanan Ratthawongjirakul
Jan 13, 2019·Letters in Applied Microbiology·G SharmaS Sethi
Mar 3, 2020·Japanese Journal of Infectious Diseases·Benjawan PhetsuksiriYasuhiko Suzuki

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