PMID: 9542919May 23, 1998Paper

Detection of Mycobacterium tuberculosis complex in sputum specimens by the automated Roche Cobas Amplicor Mycobacterium Tuberculosis Test

Journal of Clinical Microbiology
I RajalahtiA Miettinen

Abstract

Three hundred twenty-four sputum specimens from 151 patients with suspected active pulmonary tuberculosis were tested for the presence of the Mycobacterium tuberculosis complex with auramine fluorochrome stain and automated PCR assay (Roche Cobas Amplicor Mycobacterium Tuberculosis Test [MTB]). The results were compared with those of the conventional Löwenstein-Jensen tube culture and the BACTEC radiometer liquid culture. A total of 76 specimens from 32 patients were culture positive for M. tuberculosis. In addition, 37 specimens from 15 patients were smear and culture positive for other Mycobacterium species but negative by the present nucleic acid amplification method and thus were not included in the comparison. Compared with culture, the sensitivities, specificities, and positive and negative predictive values for acid-fast smear were 67, 98, 93, and 91% and those for the Cobas Amplicor MTB were 83, 99, 97, and 95%, respectively. When three consecutive sputum specimens per patient could be obtained, the sensitivity of the Cobas Amplicor MTB improved to 91%, whereas the sensitivity of the acid-fast smear remained unchanged.

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Citations

May 6, 1999·Current Opinion in Pulmonary Medicine·S Gillespie
Jan 11, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·I C ShamputaF Portaels
Dec 17, 2010·Tropical Medicine & International Health : TM & IH·Suheir EreqatZiad Abdeen
Apr 6, 2011·The Kaohsiung Journal of Medical Sciences·Chih-Bin LinJen-Jyh Lee
Aug 31, 2012·Journal of Clinical Microbiology·Yukio HidaMasayuki Iwano
Jun 14, 2012·Journal of Clinical Microbiology·Enrico TortoliDaniela M Cirillo
Sep 29, 2000·Lancet·P AndersenT M Doherty
Nov 6, 2004·Journal of Clinical Microbiology·Takayuki WadaKazuo Kobayashi
Jul 5, 2003·Journal of Clinical Microbiology·Olga L SarmientoWilliam C Miller
Dec 10, 2003·Journal of Clinical Microbiology·Claudio Piersimoni, Claudio Scarparo
Jun 29, 1999·Analytical Chemistry·C L Mouritsen, D R Hillyard

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