PMID: 9542936May 23, 1998Paper

Detection of Mycobacterium tuberculosis complex in cattle by PCR using milk, lymph node aspirates, and nasal swabs

Journal of Clinical Microbiology
F VitaleS Caracappa

Abstract

The PCR technique was applied to the diagnosis of tuberculosis in live cattle, and both skin-test-negative and skin-test-positive animals were studied. DNA was taken from various sources including specimens of lymph node aspirates, milk, and nasal swabs. After slaughter and visual inspection, tissues such as lymph nodes, lungs, and udders from tuberculin reactors were tested by the same technique. Specific oligonucleotide primers internal to the IS6110 insertion element were used to amplify a 580-bp fragment. A 182-bp fragment was obtained by designating a nested PCR from the first amplification product. This fragment was cloned and sequenced, and after being labeled it was employed in dot blot hybridization. A total of 100 cattle were tested, and PCR analysis was performed using nasal swab, milk, and lymph node aspirate. Sixty skin-test-positive cows were also tested to detect mycobacterial DNA in tissue samples from lymph nodes, lungs, and udders, and the infection was confirmed in all of the animals. Using PCR analysis of tissue samples from slaughtered animals as a "gold standard" we calculated 100% values for sensitivity, specificity, and positive and negative predictive values for milk and lymph node aspirate samples. The...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Nov 1, 1991·The American Review of Respiratory Disease·K D EisenachJ T Crawford
May 1, 1990·The Journal of Infectious Diseases·K D EisenachJ T Crawford
Sep 1, 1990·Journal of Clinical Microbiology·C C PaoC H Chang
Dec 1, 1995·Journal of Clinical Microbiology·R SzewzykG Källenius

❮ Previous
Next ❯

Citations

Jul 30, 2003·Memórias do Instituto Oswaldo Cruz·Clarice Q Fujimura LeiteRobert C Cooksey
Jul 26, 2012·International Journal of Environmental Research and Public Health·Blessing SilaigwanaRoland N Ndip
Apr 6, 2001·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·M C AntognoliT Keefe
Jun 11, 2011·Journal of Avian Medicine and Surgery·Giovanni LanteriGiuseppe Mazzullo
Jan 31, 2012·Foodborne Pathogens and Disease·Martín José ZumárragaAngel Adrián Cataldi
Jun 11, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Martín J ZumárragaAngel Cataldi
Sep 27, 2013·PLoS Neglected Tropical Diseases·Suheir EreqatGila Kahila Bar-Gal
Feb 18, 2009·Veterinary Research·Angel H AlvarezMario Alberto Flores-Valdez
Jun 22, 2012·Veterinary Medicine International·Ashutosh WadhwaShigetoshi Eda
May 8, 2015·Journal of Food Protection·André Vinícius Andrade BezerraFabiana Quoos Mayer
Jun 8, 2017·Revista Do Instituto De Medicina Tropical De São Paulo·Carmen Alicia Daza BolañosMárcio Garcia Ribeiro
Jan 28, 2017·Research in Veterinary Science·Roberta J MagnusonMo D Salman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.