Detection of Mycobacterium tuberculosis in AFB smear-negative sputum specimens through MTB culture and GeneXpert® MTB/RIF assay

International Journal of Immunopathology and Pharmacology
G RasoolMuhammad Riaz

Abstract

Tuberculosis (TB) is an important public health issue around the globe which is a chronic infectious disease and is still one of the major challenges for developing countries. The emergence of drug-resistant TB makes the condition worse and there is an urgent need of fast, highly sensitive diagnostic methods. This study was undertaken to evaluate the performance of GeneXpert® MTB/RIF assay and MTB culture for the detection of Mycobacterium tuberculosis (MTB) in sputum smear-negative pulmonary TB/drug-resistant tuberculosis (DR-TB) suspects. A total of 168 sputum smear-negative TB suspects were recruited for the study. Among the suspected TB cases, 52.98% were male and 47.02% were females with the mean age of 42 ± 17.6 years. All the sputum specimens collected from the study population were subjected to Ziehl-Neelsen (ZN) smear microscopy, GeneXpert MTB/RIF assay, and MTB culture. The results revealed that, out of 168 acid-fast bacilli (AFB)/ZN smear microscopy-negative sputum specimens, 48 (28.57%) and 58 (34.52%) were detected MTB positive by GeneXpert MTB/RIF assay and MTB culture, respectively, while 120 (71.43%) and 110 (65.48%) suspected TB cases were confirmed negative by GeneXpert MTB/RIF assay and MTB culture, respectiv...Continue Reading

References

Sep 10, 2010·The New England Journal of Medicine·Catharina C BoehmeMark D Perkins
Apr 14, 2016·International Journal of Immunopathology and Pharmacology·Muhammad RiazSyed Ehtisham-Ul-Haque
May 25, 2016·Journal of Clinical Tuberculosis and Other Mycobacterial Diseases·Adam J Caulfield, Nancy L Wengenack

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Citations

Jan 3, 2020·PloS One·Berhane Megerssa EresoMette Sagbakken
Nov 27, 2020·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Hong ZhengYanqin Shen
Dec 1, 2020·The New England Journal of Medicine·Philippe ArmandMelissa Krystel-Whittemore
Jan 13, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Wenlong ZhuWeibing Wang

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Methods Mentioned

BETA
PCR
nucleic acid amplification

Software Mentioned

GeneXpert
SPSS

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