Detection of Mycobacterium tuberculosis pncA Mutations by the Nipro Genoscholar PZA-TB II Assay Compared to Conventional Sequencing

Antimicrobial Agents and Chemotherapy
Melisa J WillbyJames E Posey

Abstract

Pyrazinamide (PZA) is a standard component of first-line treatment regimens for Mycobacterium tuberculosis and is included in treatment regimens for drug-resistant M. tuberculosis whenever possible. Therefore, it is imperative that susceptibility to PZA be assessed reliably prior to the initiation of therapy. Currently available growth-based PZA susceptibility tests are time-consuming, and results can be inconsistent. Molecular tests have been developed for most first-line antituberculosis drugs; however, a commercial molecular test is not yet available for rapid detection of PZA resistance. Recently, a line probe assay, the Nipro Genoscholar PZA-TB II assay, was developed for the detection of mutations within the pncA gene, including the promoter region, that are likely to lead to PZA resistance. The sensitivity and specificity of this assay were evaluated by two independent laboratories, using a combined total of 249 strains with mutations in pncA or its promoter and 21 strains with wild-type pncA Overall, the assay showed good sensitivity (93.2% [95% confidence interval, 89.3 to 95.8%]) and moderate specificity (91.2% [95% confidence interval, 77.0 to 97.0%]) for the identification of M. tuberculosis strains predicted to be ...Continue Reading

References

Mar 5, 2008·Antimicrobial Agents and Chemotherapy·Pontus JuréenSven Hoffner
Jul 20, 2011·Antimicrobial Agents and Chemotherapy·Kwok Chiu ChangYing Zhang
Jun 26, 2014·The Journal of Antimicrobial Chemotherapy·X GonzaloJ Werngren
Jan 27, 2015·American Journal of Respiratory and Critical Care Medicine·Andreas H DiaconCarl M Mendel
Jun 17, 2015·Antimicrobial Agents and Chemotherapy·Sarah M Ramirez-Busby, Faramarz Valafar
Sep 18, 2015·Journal of Clinical Microbiology·Nontuthuko E ManingiP Bernard Fourie
Jun 8, 2017·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M DriesenL Rigouts

❮ Previous
Next ❯

Citations

Jun 14, 2019·Journal of Clinical Microbiology·Kingsley King-Gee TamWing-Cheong Yam
Mar 20, 2021·The European Respiratory Journal·Sagal MohamedScott K Heysell
Jun 29, 2021·Antimicrobial Agents and Chemotherapy·Jim WerngrenLina Davies Forsman
Aug 21, 2018·Annual Review of Medicine·Kristen V Dicks, Jason E Stout
Oct 5, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Johanna KuhlinJudith Bruchfeld

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antitubercular Agents

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antitubercular Agents (ASM)

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.