PMID: 9650949Jul 3, 1998Paper

Detection of Mycoplasma hyopneumoniae in bronchoalveolar lavage fluids of pigs by PCR

Journal of Clinical Microbiology
A K BaumeisterH Kirchhoff

Abstract

In the present investigation we developed a method for the detection of Mycoplasma hyopneumoniae in bronchoalveolar lavage fluid (BALF) of pigs by PCR with a primer pair flanking a DNA fragment of 853 bp specific for M. hyopneumoniae. Several methods were tested to eliminate the amplification inhibitors present in BALFs. The best results were obtained by the extraction of the DNA from the BALFs. By the PCR performed with the extracted DNA, 10(2) CFU of M. hyopneumoniae could be detected in 1 ml of BALF from specific-pathogen-free swine experimentally inoculated with M. hyopneumoniae. DNA from 11 other mycoplasma species and 17 cell-walled bacterial species colonizing the respiratory tracts of pigs was not amplified. In a field study BALFs from 40 pigs from farms with a history of chronic pneumonia were tested for M. hyopneumoniae by cultivation and by PCR (i) with BALFs incubated in Friis medium and (ii) with DNA extracted from the BALFs. In addition, PCR was performed with postmortem lung washings from 19 of the 40 pigs, and immunofluorescence tests were carried out with sections of lungs from 18 of the 40 pigs. M. hyopneumoniae could not be detected in 18 of the 40 pigs by any of the five methods tested. The remaining 22 pigs...Continue Reading

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Citations

Nov 9, 2002·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kathy T KurthF Chris Minion
Apr 27, 2007·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Hugh Y CaiGrant Maxie
Aug 12, 2014·Toxicology and Industrial Health·Roland PósaTibor Magyar
Dec 20, 2002·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·M J MombargJ H M Verheijden
Jan 27, 2006·Veterinary Research Communications·T StakenborgD Maes
Sep 6, 2005·The Journal of Veterinary Medical Science·Yukiko OtagiriShizuo Sato
Oct 11, 2005·Veterinary Research Communications·P AssunçãoJ B Poveda
May 30, 2002·Veterinary Microbiology·Joachim SpergserRenate Rosengarten
Apr 4, 2000·Journal of Clinical Microbiology·J HondaK Oizumi
Mar 20, 2010·The Veterinary Journal·Mari HeinonenKatri Levonen

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