Detection of Mycoplasma pneumoniae in simulated clinical samples by polymerase chain reaction. Brief report

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
J S JensenK Lind

Abstract

Polymerase chain reaction (PCR) was used to detect Mycoplasma (M) pneumoniae DNA in simulated clinical samples. Throat swabs were mixed with known amounts of broth-grown M. pneumoniae cells. An estimated detection limit of less than 40 colony forming units (cfu) was obtained without the need for time-consuming hybridization. The PCR is completed in one day and may be useful for the early detection of M. pneumoniae in clinical samples.

References

Dec 1, 1988·The Journal of Infectious Diseases·G SchochetmanW K Jones
Nov 1, 1988·Journal of Clinical Microbiology·J B BasemanD L Rose
Dec 1, 1984·Journal of Clinical Microbiology·K LindC Christiansen

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Citations

May 1, 1994·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·F J van KuppeveldW J Melchers
Nov 1, 1993·International Journal of Antimicrobial Agents·H E Akalin
Feb 20, 2004·Diagnostic Microbiology and Infectious Disease·Merete StorgaardJørgen S Jensen
Feb 14, 1992·Journal of Immunological Methods·V KamlaU Hadding
Jul 26, 2003·Seminars in Pediatric Infectious Diseases·Ari BitnunSusan Richardson
May 22, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A BitnunS Richardson
Nov 8, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jørgen S JensenRyoichi Hamasuna
May 1, 1991·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·L Hirschberg, T Holme
Oct 19, 2004·Clinical Microbiology Reviews·Ken B Waites, Deborah F Talkington
Nov 8, 2003·Journal of Clinical Microbiology·K LoensM Ieven
Sep 2, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·John S BradleyUNKNOWN Pediatric Infectious Diseases Society and the Infectious Diseases Society of America
Apr 28, 2007·American Journal of Public Health·Lisa E ManhartPatricia A Totten
Jun 1, 1996·Clinical Otolaryngology and Allied Sciences·P A GurrS J Gurr
Jul 30, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Christoph P BergGerburg M Stein
Apr 26, 2008·Pediatric Pulmonology·Birgitte B KjaerHans Bisgaard
Sep 16, 2005·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Miyuki MorozumiKimiko Ubukata
Jun 1, 1991·The Journal of Hospital Infection·M J Pallen, P D Butcher
Aug 30, 2008·FEMS Microbiology Reviews·Thomas Prescott AtkinsonKen B Waites
Aug 20, 2015·BMC Infectious Diseases·Jørgen Skov Jensen, Catriona Bradshaw
Apr 24, 2007·The Journal of Adolescent Health : Official Publication of the Society for Adolescent Medicine·Aneesh K ToshDonald P Orr
May 26, 2017·Clinical Microbiology Reviews·Ken B WaitesT Prescott Atkinson
Feb 1, 1993·Letters in Applied Microbiology·N A ZigangirovaS V Prozorovsky
May 1, 1996·The Journal of Applied Bacteriology·H HotzelH Pfützner
Mar 1, 1994·The Journal of Applied Bacteriology·O V NorkinaI G Drosdov
Dec 1, 1992·Journal of Clinical Microbiology·G E BuckJ T Summersgill
May 23, 1998·Journal of Clinical Microbiology·D F TalkingtonJ S Jensen
Mar 1, 1994·Applied and Environmental Microbiology·O Dussurget, D Roulland-Dussoix

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