Detection of MYD88 and CXCR4 mutations in cell-free DNA of patients with IgM monoclonal gammopathies

Leukemia
Tina BagratuniMeletios A Dimopoulos

Abstract

Liquid biopsyis being integrated into cancer diagnostics with profound therapeutic implications. However, its role in Waldenström's Macroglobulinemia (WM) and IgM monoclonal gammopathies is still unclear. In this study, we evaluated the role of peripheral blood (PB) cell-free DNA (cfDNA) in characterizing the mutational status of MYD88 and CXCR4 of patients with IgM monoclonal gammopathies. Paired bone marrow (BM) tumor DNA (tDNA) and PB cfDNA samples from 98 patients (9 MGUS, 45 with WM in remission, 44 with smoldering WM, newly diagnosed or relapsed WM) and 10 controls with non-IgM monoclonal gammopathies were analyzed. Regarding MYD88L265P mutation, 76 patients had paired tDNA and cfDNA informative samples. Among patients with WM in remission, 65% harbored the MYD88L265P mutation, whereas the corresponding percentage among smoldering/newly diagnosed or relapsed WM was 92%. The overall concordance rate was 94% (72/76). For CXCR4 mutations, 65 patients had paired informative tDNA and cfDNA samples. The overall concordance rate was 90% (59/65). All controls had wild-type MYD88 and CXCR4. In conclusion, PB cfDNA is a useful, minimally invasive, cost-effective, and time-effective tool for the identification of the presence of MYD...Continue Reading

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Citations

Feb 23, 2020·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Steven P TreonJorge J Castillo
Apr 9, 2020·Vaccines·Stefania ScalaElena Monica Borroni
Jul 8, 2020·Expert Review of Anticancer Therapy·Maria GavriatopoulouMeletios Athanasios Dimopoulos
Jul 21, 2020·Journal of the Peripheral Nervous System : JPNS·Chiara BrianiAngelo Quattrini
Nov 7, 2019·Medicine·Maria GavriatopoulouMeletios-Athanasios Dimopoulos
Sep 22, 2020·Expert Opinion on Emerging Drugs·Fotiou DespinaKastritis Efstathios
Aug 8, 2020·Expert Review of Anticancer Therapy·Ioannis Ntanasis-StathopoulosTimothy M Pawlik
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Jul 30, 2020·Clinical Lymphoma, Myeloma & Leukemia·Ioannis Ntanasis-StathopoulosMeletios A Dimopoulos
Dec 25, 2019·Seminars in Cancer Biology·Sara K DanielVenu G Pillarisetty
Feb 12, 2021·Leukemia & Lymphoma·Andrew R BranaganJorge J Castillo
Feb 23, 2021·Therapeutic Advances in Hematology·Ioannis Ntanasis-StathopoulosMeletios A Dimopoulos
Apr 6, 2021·American Journal of Hematology·Maria G DemosJoshua N Gustine
Jun 26, 2021·Expert Review of Hematology·Ilias PessachEfstathios Kastritis
Nov 18, 2021·Blood Advances·Tina BagratuniMeletios A Dimopoulos

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Methods Mentioned

BETA
biopsy
PCR
electrophoresis
bone marrow aspiration

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