Detection of myo-inositol trispyrophosphate in equine urine and plasma by hydrophillic interaction chromatography-tandem mass spectrometry

Drug Testing and Analysis
April S Y WongTerence S M Wan

Abstract

Myo-inositol trispyrophosphate (ITPP) is a new drug capable of increasing the amount of oxygen in hypoxic tissues. Studies have shown that administration of ITPP increases the maximal exercise capacity in normal mice as well as mice with severe heart failure. The properties of ITPP make it an ideal candidate as a doping agent to enhance performance in racehorses. While there have been speculations in the horseracing industry that the covert use of ITPP is already widespread, no reported method exists for the detection of ITPP in equine biological samples. ITPP is a difficult-to-detect drug due to its hydrophilic nature; the complexity of equine biological matrices also adds to the problem. This paper describes for the first time a method for the detection and confirmation of ITPP in equine urine and plasma. ITPP was isolated from the sample matrices by solid-phase extraction and the extract was analyzed by hydrophilic interaction chromatography-tandem mass spectrometry. ITPP could be detected at low ppb levels in both fortified equine plasma and urine with good precision, fast instrumental turnaround time, and negligible matrix interferences. To our knowledge, this is the first report of a validated method for the detection and...Continue Reading

References

Mar 5, 2005·Bioorganic & Medicinal Chemistry Letters·Konstantina C FylaktakidouClaude Nicolau
Oct 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Claudine KiedaClaude Nicolau
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Citations

Jun 5, 2013·Drug Testing and Analysis·Geoffrey LamBarbara Morrissey
Jan 15, 2017·Drug Testing and Analysis·A G FragkakiM Tsivou
Nov 17, 2012·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Brigitte GerinHugues Chanteux
Mar 8, 2017·Drug Testing and Analysis·Adam T Cawley, John Keledjian
Jan 14, 2021·Drug Testing and Analysis·Fernando MoreiraMaria de Lourdes Bastos

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