Detection of myocardial ischemia-reperfusion injury using a fluorescent near-infrared zinc(II)-dipicolylamine probe and 99mTc glucarate.

Molecular Imaging
Leonie WyffelsZhonglin Liu

Abstract

A fluorescent zinc 2,2'-dipicolylamine coordination complex PSVue®794 (probe 1) is known to selectively bind to phosphatidylserine exposed on the surface of apoptotic and necrotic cells. In this study, we investigated the cell death targeting properties of probe 1 in myocardial ischemia-reperfusion injury. A rat heart model of ischemia-reperfusion was used. Probe 1, control dye, or 99mTc glucarate was intravenously injected in rats subjected to 30-minute and 5-minute myocardial ischemia followed by 2-hour reperfusion. At 90 minutes or 20 hours postinjection, myocardial uptake was evaluated ex vivo by fluorescence imaging and autoradiography. Hematoxylin-eosin and cleaved caspase-3 staining was performed on myocardial sections to demonstrate the presence of ischemia-reperfusion injury and apoptosis. Selective accumulation of probe 1 could be detected in the area at risk up to 20 hours postinjection. Similar topography and extent of uptake of probe 1 and 99mTc glucarate were observed at 90 minutes postinjection. Histologic analysis demonstrated the presence of necrosis, but only a few apoptotic cells could be detected. Probe 1 selectively accumulates in myocardial ischemia-reperfusion injury and is a promising cell death imaging ...Continue Reading

References

Jul 1, 1997·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·B A KhawJ Narula
Sep 2, 1999·Journal of Molecular and Cellular Cardiology·T A HollyV L Cryns
Mar 23, 2000·Cardiovascular Research·A Saraste, K Pulkki
Mar 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Allen M Gown, Mark C Willingham
Oct 29, 2002·Journal of Clinical Pathology·P A J KrijnenH W M Niessen
Jan 13, 2004·American Journal of Physiology. Heart and Circulatory Physiology·James D McCullySidney Levitsky
Aug 18, 2004·Apoptosis : an International Journal on Programmed Cell Death·F GaoX L Ma
May 26, 2005·Bioorganic & Medicinal Chemistry·Roger G Hanshaw, Bradley D Smith
May 12, 2006·Organic & Biomolecular Chemistry·Kristy M DiVittorioBradley D Smith
Dec 21, 2006·Journal of the American Chemical Society·W Matthew LeevyBradley D Smith
Apr 5, 2007·Nature Protocols·Hugo van GenderenChris Reutelingsperger
Mar 4, 2008·Methods : a Companion to Methods in Enzymology·Sabine JakobThomas Brunner
Jul 1, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Francis G Blankenberg
Nov 26, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Aude BressenotFrançois Plénat
Apr 14, 2009·Methods : a Companion to Methods in Enzymology·Marijke De Saint-HubertFelix M Mottaghy
Feb 4, 2010·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Heidi KenisChris P M Reutelingsperger
Feb 13, 2010·Annual Review of Physiology·Russell S WhelanRichard N Kitsis
Sep 2, 2010·Bioconjugate Chemistry·Geralda A F van TilborgKlaas Nicolay
Apr 16, 2011·Nuclear Medicine and Biology·Matthias BauwensAlfons Verbruggen

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Citations

Feb 9, 2019·Scientific Reports·Francesca MazzoniSilvia C Finnemann
Mar 10, 2020·Bioconjugate Chemistry·Dongjian ZhangJian Zhang

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Methods Mentioned

BETA
fluorescence imaging
fluorescence
xenografts

Software Mentioned

FujiFilm Multi - Gauge
Olympus CellSense Digital Imaging
ImageJ

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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