Detection of N-hydroxy-2-acetylaminofluorene-DNA adducts in rat liver measured by radioimmunoassay

Carcinogenesis
P RioM Leng

Abstract

Antibodies to imidazole ring opened derivatives of alkali treated N-(guanosin-8-yl)-2-aminofluorene (1-[6-(2,5-diamino-4-oxopyrimidinyl-N6-riboside)]-3-(2-fluorenyl)urea were elicited in rabbits by immunization with a conjugate between the ring-opened derivative and bovine serum albumin. The specificity of the antibodies was studied by radioimmunoassay. These antibodies and antibodies to N-(guanosin-8-yl)-N-acetyl-2-aminofluorene were used to titrate the adducts formed in liver DNA of rats treated with N-hydroxy-2-acetylaminofluorene. The ring-opened derivatives represent approximately 10% of the dGuo-C8-adducts.

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.