PMID: 2496754Feb 7, 1989Paper

Detection of nearest neighbors to specific fluorescently tagged ligands in rod outer segment and lymphocyte plasma membranes by photosensitization of 5-iodonaphthyl 1-azide

Biochemistry
Y RavivY Salomon

Abstract

Lima bean agglutinin-fluorescein 5-isothiocyanate conjugate (FluNCS-lima bean lectin) interacts with specific receptor molecules on membranes both from the rod outer segment (ROS) of the frog retina and from S49 mouse lymphoma cells. When [125I]-5-iodonaphthyl 1-azide (125I-INA), which freely and randomly partitions into the lipid bilayer, is added to membranes and the suspension is irradiated at 480 nm, the FluNCS-conjugated lectin photosensitizes the [125I]INA but only at discrete sites. This results in the selective labeling of specific proteins: an 88-kDa protein on ROS membranes and a 56-kDa protein on S49 plasma membranes. Labeling is dependent upon the interaction of the FluNCS-lectin with glycosylated receptor sites, since N-acetylgalactosamine, but not methyl alpha-mannoside, blocked labeling of the 56-kDa protein on S49 membranes. In contrast, a random labeling pattern of membrane proteins was observed upon irradiation at 480 nm using other fluorescein conjugates, such as FluNCS-bovine serum albumin (FluNCS-BSA) or FluNCS-soybean trypsin inhibitor (FluNCS-STI), which interact with cell membranes in a nonselective manner, or with N-(fluorescein-5-thiocarbamoyl)-n-undecyclamine (FluNCS-NHC11), which is freely miscible i...Continue Reading

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Citations

Mar 1, 1991·Chemistry and Physics of Lipids·K Lohner
Jul 12, 2003·Journal of Biomolecular Structure & Dynamics·Dmitry M Kolpashchikov
Jan 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jing LiArthur Karlin
Jan 1, 1995·Molecular Membrane Biology·R BlumenthalR J Lowy
Oct 10, 1993·Biochimica Et Biophysica Acta·K LohnerF Paltauf
Sep 22, 2021·Chemical Reviews·Vivian M LechnerMatthew J Gaunt

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