Detection of new cry genes of Bacillus thuringiensis by use of a novel PCR primer system.

Applied and Environmental Microbiology
Pedro A Noguera, Jorge E Ibarra

Abstract

On the basis of the known cry gene sequences of Bacillus thuringiensis, three sets of primers were designed from four conserved blocks found in the delta-endotoxin-coding region. The primer pairs designed amplify the regions between blocks 1 and 5, 2 and 5, and 1 and 4. In silico analyses indicated that 100% of the known three-domain cry gene sequences can be amplified by these sets of primers. To test their ability to amplify known and unknown cry gene sequences, 27 strains from the CINVESTAV (LBIT series) collection showing atypical crystal morphology were selected. Their DNA was used as the template with the new primer system, and after a systematic amplification and sequencing of the amplicons, each strain showed one or more cry-related sequences, totaling 54 different sequences harbored by the 27 strains. Seven sequences were selected on the basis of their low level of identity to the known cry sequences, and once cloning and sequencing of the complete open reading frames were done, three new cry-type genes (primary ranks) were identified and the toxins that they encode were designated Cry57Aa1, Cry58Aa1, and Cry59Aa1 by the B. thuringiensis Toxin Nomenclature Committee. The rest of the seven sequences were classified Cry8...Continue Reading

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Citations

Jul 25, 2012·Cell Biochemistry and Biophysics·Annapurna S AgasthyaPrabhpreet Mahal
Sep 27, 2014·World Journal of Microbiology & Biotechnology·Wenfei ZhangXuanjun Fang
Sep 19, 2015·Letters in Applied Microbiology·A I Sánchez-SotoM C Del Rincón-Castro
Dec 6, 2011·Journal of Invertebrate Pathology·Wenfei ZhangXuanjun Fang
Dec 22, 2016·Environmental Science & Technology·Anderson Abel de Souza MachadoWerner Kloas
Mar 12, 2017·Toxins·Rooma AdalatAbdul Rauf Shakoori
Jan 10, 2019·3 Biotech·J Francisco Castillo-EsparzaJorge E Ibarra
Nov 28, 2017·Applied Microbiology and Biotechnology·Bhupendra S PanwarSarvjeet Kaur
Jan 26, 2021·International Journal of Biological Macromolecules·Drishtant SinghAnup Kumar Kesavan
Sep 8, 2021·Antonie van Leeuwenhoek·J Francisco Castillo-EsparzaJorge E Ibarra

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