PMID: 8601693Apr 1, 1996Paper

Detection of nitric oxide synthase (NOS) in somatostatin-producing cells of human and murine stomach and pancreas

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
M A BurrellA C Villaro

Abstract

The aim of this study was to identify by immunocytochemistry the distribution of nitric oxide synthase (NOS) in human and murine gastric epithelium. Using two different antisera specific for neuronal NOS (nNOS), we detected nNOS immunoreactivity in endocrine cells of the epithelium of the body and pyloric regions as well as in ganglion cells of the intrinsic plexi of the stomach of the three species studied. Both immunocytochemistry of contiguous sections and double immunolabeling methods showed that the nNOS-immunoreactive cells were also immunoreactive for somatostatin. Co-localization of nNOS and somatostatin has also been found in the pancreatic islets, where strong nNOS immunoreactivity appeared in scattered cells, which were peripheral in rat and mouse islets and more randomly distributed in human. The possibility of crossreactivity between the antisera against nNOS and somatostatin was ruled out by means of absorption controls. Immunocytochemical techniques were also applied to thin sections, confirming the immunostaining of gastric D-cells, which was restricted principally to the secretory granules. The possible functional implications of these findings for gastric and pancreatic physiology are discussed.

Citations

Dec 3, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M ChvanovA Tepikin
May 22, 2003·Progress in Histochemistry and Cytochemistry·Luis M MontuengaAna C Villaro
Oct 27, 2016·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·A J Page, S J Kentish
May 29, 2013·Scientific Reports·Igor BuchwalowWerner Boecker
Aug 12, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·T M Fonovich de SchroederO L Catanzaro
Oct 17, 1998·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Onoda, H Inano
Jun 15, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Marta ArchancoMaría A Burrell
Jul 18, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M García-VitoriaM A Burrell
Sep 22, 2006·Cell Proliferation·I R Radford, P N Lobachevsky
Jan 5, 2002·Canadian Journal of Physiology and Pharmacology·V C SmithA M Buchan
Feb 24, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·S PremaratneM L Schubert
Jan 17, 2019·Acta Histochemica Et Cytochemica·Igor BuchwalowKatharina Tiemann
Oct 23, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·T KiviluotoE Kivilaakso
Feb 1, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B H CaoL I Larsson
May 8, 1999·Biochimica Et Biophysica Acta·E Rodríguez-MartínE Arilla

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