PMID: 11930111Apr 4, 2002Paper

Detection of nodal micrometastases using immunohistochemistry and PCR in melanoma of the arm and trunk

Melanoma Research
S BoiP Dalla Palma

Abstract

Sentinel node (SN) mapping and biopsy seems at present the best way to assess the nodal status in cutaneous melanoma without removing the lymphatic chain. The procedure is minimally invasive, safe and low cost, and allows selection of patients who can benefit from elective node dissection. From March 1997 up to July 1999 we examined 112 SNs excised after lymphatic mapping from 95 patients (48 males and 47 females) with stage I cutaneous melanoma affecting the trunk or limbs. Of these, 88 SNs from 74 patients were submitted to polymerase chain reaction (PCR) in order to detect tyrosinase mRNA. A new antibody (anti-tyrosinase, Clone T311, IgG2a type, Lab Vision Corporation) was used to detect nodal micrometastases. The search for micrometastases was histologically positive in 15 SNs and negative in 97. The 88 SNs examined using molecular biology were positive in 40 cases and negative in 48. In 28 only the PCR was positive. The new antibody used to detect micrometastases was shown to be very useful. Cases positive on both conventional histology and PCR were Clark level II or more and were thicker than 0.6 mm. No difference with regard to site or sex was observed. Lymphoedema and hypersensitivity reactions, nor the inability to wor...Continue Reading

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Citations

Jan 29, 2008·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Valeria C DenninghoffBoris Elsner
Apr 24, 2004·The American Journal of Surgical Pathology·Shaofeng Yan, Jeoffry B Brennick
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Aug 14, 2008·Seminars in Diagnostic Pathology·Jeoffry B Brennick, Shaofeng Yan
Apr 25, 2006·Surgical Oncology Clinics of North America·Steve R MartinezDave S B Hoon
Apr 20, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Simone MocellinDonato Nitti
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May 13, 2005·Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology·Valeria C DenninghoffBoris Elsner

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