Detection of non-polio enteroviruses from 17 years of virological surveillance of acute flaccid paralysis in the Philippines.

Journal of Medical Virology
Lea Necitas G ApostolHitoshi Oshitani

Abstract

Acute flaccid paralysis (AFP) surveillance has been conducted as part of the World Health Organization (WHO) strategy on poliomyelitis eradication. Aside from poliovirus, which is the target pathogen, isolation, and identification of non-polio enteroviruses (NPEVs) is also done by neutralization test using pools of antisera which can only identify limited number of NPEVs. In the Philippines, despite the significant number of isolated NPEVs, no information is available with regard to its occurrence, diversity, and pattern of circulation. In this study, a total of 790 NPEVs isolated from stool samples submitted to the National Reference Laboratory from 1992 to 2008 were analyzed; neutralization test was able to type 55% (442) of the isolates. Of the remaining 356 isolates, which were untyped by using neutralization test, 348 isolates were analyzed further by RT-PCR targeting the VP1 gene. A total of 47 serotypes of NPEV strains were identified using neutralization test and molecular typing, including 28 serotypes of human enterovirus B (HEV-B), 12 serotypes of HEV-A, and 7 of HEV-C. The HEV-B group (625/790; 79%) constituted the largest proportion of isolates, followed by HEV-C (108/790; 13.7%), HEV-A (57/790; 7.2%), and no HEV-D...Continue Reading

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Citations

Jan 25, 2013·Emerging Infectious Diseases·Harjeet Singh MaanTapan N Dhole
Aug 22, 2014·BMC Infectious Diseases·Jagar A JasemK M Monirul Islam
Dec 29, 2013·Journal of Virology·Tadatsugu ImamuraHitoshi Oshitani
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Dec 30, 2014·Virus Genes·Zaharoula KyriakopoulouPanayotis Markoulatos
Dec 25, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Shahzad ShaukatSyed Sohail Zahoor Zaidi
May 3, 2019·BMC Infectious Diseases·Lea Necitas ApostolHitoshi Oshitani
Feb 6, 2020·BMC Public Health·Maria Melissa Ann JiaoAmado Tandoc
Dec 3, 2016·Human Vaccines & Immunotherapeutics·Ivanildo P SousaEdson Elias da Silva
Jun 28, 2021·Physical Medicine and Rehabilitation Clinics of North America·William IdeMelissa K Trovato

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Methods Mentioned

BETA
Partial Sequencing
PCR
electrophoresis

Software Mentioned

Clustal
MEGA

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