Detection of non-steroidal anti-inflammatory drugs in equine plasma and urine by gas chromatography-mass spectrometry

Journal of Chromatography. a
G GonzálezJ Segura

Abstract

A gas chromatographic-mass spectrometric (GC-MS) procedure for the detection of seventeen non-steroidal anti-inflammatory drugs (NSAIDs) in equine plasma and urine samples is described. The extraction of the compounds from the biological matrix was performed at acidic pH (2-3) with diethyl ether. Ethereal extracts were washed with a saturated solution of sodium hydrogencarbonate (urine) or treated with a solid mixture of sodium carbonate and sodium hydrogencarbonate (plasma). The ethereal extracts were dried and derivatized by incubation at 60 degrees C with methyl iodide in acetone in the presence of solid potassium carbonate. Mono- or bismethyl derivatives of the NSAIDs were obtained. After derivatization kinetic studies, 90 min was the incubation time finally chosen for screening purposes for adequate methylation of all the compounds under study. For individual confirmation analyses, shorter incubation times can be used. The chromatographic analysis of the derivatives was accomplished by GC-MS with a run time of 13 min. In general, extraction recoveries ranged from 23.3 to 100% in plasma and from 37.5 to 83.8% in urine samples. Detection limits from less than 5 to 25 ng/ml were obtained for both plasma and urine samples usin...Continue Reading

References

Jun 10, 1992·Journal of Chromatography·M C RouanJ Godbillon
Jan 1, 1989·Journal of Pharmaceutical and Biomedical Analysis·E G de JongR A Maes
May 13, 1988·Journal of Chromatography·M Johansson, E L Anlér
Sep 1, 1993·Journal of Veterinary Pharmacology and Therapeutics·F T DelbekeM Debackere

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Citations

Jul 4, 2012·Journal of Fluorescence·J A Murillo PulgarínF Martínez Ferreras
Nov 7, 2001·Journal of Veterinary Pharmacology and Therapeutics·J Y KimB C Chung
Aug 15, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·G DowlingL Regan
Sep 15, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Koichi SuenamiSusumu Kanno
Feb 20, 2007·Journal of Separation Science·Koichi SuenamiSusumu Kanno
Sep 29, 2009·Journal of Chromatography. a·Brian KinsellaMartin Danaher

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