PMID: 9542938May 23, 1998Paper

Detection of Norwalk virus and other genogroup 1 human caliciviruses by a monoclonal antibody, recombinant-antigen-based immunoglobulin M capture enzyme immunoassay

Journal of Clinical Microbiology
J P BrinkerJ E Herrmann

Abstract

Sera obtained from two groups of adult volunteers infected with Norwalk virus (NV) and two groups of patients involved in two natural outbreaks were tested for NV-reactive immunoglobulin M (IgM) by use of a monoclonal antibody, recombinant-antigen-based IgM capture enzyme immunoassay (EIA). No NV-reactive IgM was detected in the preinoculation sera of 15 volunteers, and 14 of 15 showed NV-reactive antibodies postinfection with NV. All of the volunteers showed IgG seroconversion to NV. In the outbreak studies, all 9 persons in one outbreak and 19 of 24 in another outbreak had NV-reactive IgM. In the first outbreak, only three of nine seroconverted to NV, which was likely due to late collection of acute-phase sera. In the second outbreak, 21 of 24 showed IgG seroconversion to NV. Sequencing of viruses isolated from five stool samples selected from those in the second outbreak showed that they were human calicivirus (HuCV) genogroup 1 viruses related, but not identical, to NV. In the volunteer studies, NV-reactive IgM was first detected 8 days postinoculation. The time of development of NV-reactive IgM antibodies in natural outbreaks was estimated to be similar to that found in the volunteer studies. Sera from three Hawaii virus-i...Continue Reading

References

Jul 25, 1991·The New England Journal of Medicine·N R Blacklow, H B Greenberg
Dec 14, 1990·Science·J N XiM K Estes
Jan 1, 1989·American Journal of Epidemiology·M L FleissnerN A Nowak
Oct 1, 1987·Journal of Clinical Microbiology·G W GaryA V Bartlett
Oct 1, 1985·Journal of Medical Virology·J E HerrmannN R Blacklow
Aug 1, 1982·Infection and Immunity·G CukorN R Blacklow
Sep 1, 1995·Journal of Clinical Microbiology·J E HerrmannJ P Brinker
Jan 1, 1995·Archives of Virology·X JiangM K Estes
Jul 1, 1994·Journal of Medical Virology·W D CubittM K Estes
Jul 1, 1994·The Journal of Infectious Diseases·D Y GrahamM K Estes
Dec 1, 1995·Journal of Medical Virology·X JiangL K Pickering
Jan 1, 1996·Archives of Virology·F Le GuyaderR L Atmar

❮ Previous
Next ❯

Citations

May 11, 2000·The Journal of Infectious Diseases·X JiangD O Matson
Mar 8, 2008·Emerging Infectious Diseases·Giacomo MiglioratiFranco Maria Ruggeri
Aug 5, 2010·Critical Care Medicine·Linda D Bobo, Erik R Dubberke
Jun 29, 2007·Journal of Medical Virology·Nobuhiro IritaniYoshiyuki Seto
Mar 20, 2015·Foodborne Pathogens and Disease·Xiaohong LiuXiaoqin Wang
May 11, 2000·The Journal of Infectious Diseases·T AndoR L Fankhauser
Aug 24, 2007·Clinical and Vaccine Immunology : CVI·Jim J GraySabine M Diedrich
Jul 1, 2002·Comprehensive Reviews in Food Science and Food Safety·A I SairL A Jaykus
Aug 17, 1999·Journal of Clinical Microbiology·J P BrinkerJ E Herrmann
Jan 10, 2002·Infectious Disease Clinics of North America·G W Procop

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.