Detection of Novel Proline 3-Hydroxylase Activities in Streptomyces and Bacillus spp. by Regio- and Stereospecific Hydroxylation of l-Proline.

Applied and Environmental Microbiology
H MoriA Ozaki

Abstract

During the screening of microbial proline hydroxylases, novel proline 3-hydroxylase activities, which hydroxylate free l-proline to free cis-3-hydroxy-l-proline, were detected in whole cells of Streptomyces sp. strain TH1 and Bacillus sp. strains TH2 and TH3 from 3,000 strains isolated from soil. The reaction product was purified from a reaction mixture of Streptomyces sp. strain TH1, and its chemical structure was identified as cis-3-hydroxy-l-proline by instrumental analyses. Proline 3-hydroxylase activity was also detected in Streptomyces canus ATCC 12647 which produces the 3-hydroxyproline-containing peptide antibiotic telomycin. Bacillus sp. strains TH2 and TH3 were found to accumulate cis-3-hydroxy-l-proline in culture media at 426 and 352 (mu)M, respectively. It was suggested that hydroxylation occurred in a highly regio- and stereospecific manner at position 3 of l-proline because no hydroxylation product other than cis-3-hydroxy-l-proline was observed. Proline 3-hydroxylases of these strains were first characterized on crude enzyme preparations. Since 2-oxoglutarate and ferrous ion were required for hydroxylation of l-proline, these 3-hydroxylases were thought to belong to a family of 2-oxoglutarate-related dioxygenase...Continue Reading

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Citations

Jul 17, 2004·Microbiology·Lionel FerrièresDaniel Kahn
Jun 20, 2013·Applied Microbiology and Biotechnology·Thi Mai Hoa Bach, Hiroshi Takagi
Jan 13, 2012·Beilstein Journal of Organic Chemistry·Christian Klein, Wolfgang Hüttel
Dec 9, 2016·Scientific Reports·Seiya WatanabeYasuo Watanabe
Apr 16, 2020·Applied Microbiology and Biotechnology·Ryotaro Hara, Kuniki Kino
Dec 12, 2001·European Journal of Biochemistry·I J CliftonC J Schofield
Sep 3, 1999·Applied and Environmental Microbiology·T ShibasakiA Ozaki
Nov 11, 2019·Bioorganic Chemistry·Tristan J SmartChristopher J Schofield
Jul 11, 2018·Applied Microbiology and Biotechnology·Feng ZhangXiangfeng Meng
Jun 5, 2015·Journal of the American Chemical Society·Chengzhang FuRolf Müller
Jan 31, 2013·Journal of Agricultural and Food Chemistry·Ying-lao ZhangJia-dong Xu
May 10, 2020·Journal of the American Society for Mass Spectrometry·Baku AcharyaAmanda L Patrick

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