Detection of numerical chromosomal abnormalities by fluorescence in situ hybridization of interphase cell nuclei with chromosome-specific probes on archival cytologic samples

Diagnostic Cytopathology
R S CajulisS Eggena

Abstract

Fluorescence in situ hybridization (FISH) is rapidly emerging as a tool for analyzing numerical and structural chromosomal abnormalities in both liquid and solid tumors. Most studies make use of fresh samples. To determine the feasibility of detecting numerical chromosomal abnormalities (NCA) by FISH using chromosome-specific probes 8, 12, 17, and X (Vysis, Inc., Downers Grove, IL) on archival cytologic preparations, we studied 23 patient samples, one Papanicolaou-and one Diff-Quik-stained slide per case (46 slides), and two additional unstained slides (fresh ascitic fluids) as controls. Included in this study were nine ascitic fluids (four benign and five malignant), four malignant pleural fluids, three benign bladder washes, and seven malignant fine-needle aspirates (FNA) from various sites. The slides ranged from 1-94 days old. After removal of coverslips using xylene, all slides were destained in a series of alcohol and water washes. Pretreatment of slides with pepsin was followed by the in situ hybridization procedure. Two hundred cells per slide were evaluated for distinct separate signals. Results showed the following: 1) all slides were evaluable except for eight (8/46) which had either too few cells or enough cells but...Continue Reading

References

Mar 1, 1989·Cancer Genetics and Cytogenetics·J A BiegelB S Emanuel
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Citations

Jun 19, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Martina MirlacherGuido Sauter
Mar 16, 2004·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Mona FahmyGary W Falk
Feb 21, 2002·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Feng Jiang, Ruth L Katz
May 28, 2003·Diagnostic Cytopathology·Ravindra VeeramachaneniElba A Turbat-Herrera
Nov 8, 2002·Cytopathology : Official Journal of the British Society for Clinical Cytology·A MezzelaniS Pilotti
Dec 10, 1999·Cytopathology : Official Journal of the British Society for Clinical Cytology·G H YuA M Horcher

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