Detection of occult tumor cells in lymph nodes from non-small cell lung cancer patients using reverse transcription-polymerase chain reaction for carcinoembryonic antigen mRNA with the evaluation of its sensitivity

Lung Cancer : Journal of the International Association for the Study of Lung Cancer
Jun MaedaNariaki Matsuura

Abstract

We evaluated the usefulness of a real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) method for detecting occult tumor cells in histologically malignant-negative lymph nodes resected from patients with non-small cell lung cancer. First, we examined the relationship between tumor cell number and carcinoembryonic antigen (CEA) mRNA copy number using a PCR method with a cancer cell line (A549) in a serial dilution study. Next, we evaluated the relationship between nodal metastatic area size and CEA mRNA copy number using lymph nodes with histologically proven metastasis in a serial slice study. On the basis of those results, we performed RT-PCR analyses with 28 primary tumors and 211 lymph nodes from 28 patients who underwent a lobectomy with systematic node dissection. Our results in the serial dilution study showed that the detectable limitation by quantitative RT-PCR was 25-100 neoplastic cells and 20-100 CEA mRNA copy numbers. In the serial slice study, we found a correlation between CEA mRNA copy number and nodal metastatic area. In the clinical samples, amplification of CEA mRNA was obtained with all 28 primary tumors and 13 of the lymph nodes with metastasis shown by hematoxylin-eosin staining. F...Continue Reading

References

Sep 1, 1996·The Journal of Thoracic and Cardiovascular Surgery·J R IzbickiK Pantel
Feb 1, 2002·Surgery·Mayumi IshidaKeizo Sugimachi
Jun 29, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Toshihiro OsakiKosei Yasumoto
Oct 16, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Shingo NouraMorito Monden
Apr 3, 2003·International Journal of Cancer. Journal International Du Cancer·Keisuke KubotaMichio Kaminishi
Jan 23, 2004·The New England Journal of Medicine·Rodrigo ArriagadaUNKNOWN International Adjuvant Lung Cancer Trial Collaborative Group
Apr 23, 2004·The New England Journal of Medicine·Harubumi KatoUNKNOWN Japan Lung Cancer Research Group on Postsurgical Adjuvant Chemotherapy
Jan 22, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Françoise Le Pimpec-BarthesJean-François Bernaudin

❮ Previous
Next ❯

Citations

Jul 24, 2012·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Kentaro AnamiKatsunobu Kawahara
Oct 13, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Valerie W RuschRichard J Cote
Jun 29, 2010·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Esther HerpelHans Hoffmann
Jan 16, 2009·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Jun MaedaNariaki Matsuura
Oct 24, 2007·Human Pathology·Matthias WoenckhausWolfgang Dietmaier
Jan 8, 2008·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Marcin Skrzypski
Dec 21, 2014·Seminars in Thoracic and Cardiovascular Surgery·Krista J Hachey, Yolonda L Colson
Jun 19, 2016·Diagnostic Pathology·Ane Kongsgaard RudGunhild Mari Mælandsmo
Mar 7, 2018·American Journal of Clinical Oncology·Aránzazu Lafuente-SanchisJosé M Galbis-Caravajal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.