Detection of okadaic acid and related esters in mussels during diarrhetic shellfish poisoning (DSP) episodes in Greece using the mouse bioassay, the PP2A inhibition assay and HPLC with fluorimetric detection

Toxicon : Official Journal of the International Society on Toxinology
Eleanna PrassopoulouApostolos Kyritsakis

Abstract

An approach involving chemical, functional and biological techniques was taken for the detection and quantification of the marine toxin okadaic acid (OA) in mussels from Thermaikos and Saronikos Gulfs, Greece, during DSP episodes that occurred in 2006-2007. Samples were analyzed using the mouse bioassay, high performance liquid chromatography with fluorimetric detection (HPLC-FLD), using l-bromoacetylpyrene (BAP), as a precolumn derivatisation reagent, and the protein phosphatase 2A inhibition assay (PP2AIA) using a commercially available kit. Okadaic acid (OA) and its polar and non-polar esters were detected and quantified by HPLC-FLD, after hydrolysis of the samples during preparation. The detection limit of the HPLC method for OA was 5.86 microg OA/kg, which permits this method to be used for the regulatory control of these toxins in shellfish. Comparison of the results by all three methods revealed excellent consistency.

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Citations

Jan 23, 2014·Marine Drugs·Beatriz RegueraJuan Blanco
Aug 12, 2014·Toxicon : Official Journal of the International Society on Toxinology·Ernesto García-MendozaJosé Luis Peña-Manjarrez
Aug 30, 2014·Environmental Science and Pollution Research International·Chao LinShi-Ying Lu
Oct 12, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Artemis P LouppisMichael G Kontominas
Oct 3, 2013·Marine Drugs·Bich-Thuy L EberhartVera L Trainer
May 12, 2010·Analytical and Bioanalytical Chemistry·Natalia VilariñoLuis M Botana
Jul 26, 2012·Environmental Science and Pollution Research International·Shi-Ying LuHong-Lin Ren
Nov 3, 2009·Toxicon : Official Journal of the International Society on Toxinology·Artemis P LouppisMichael G Kontominas
Nov 10, 2014·Analytical and Bioanalytical Chemistry·Karen L BruceClaire E Lenehan
May 31, 2018·Marine Drugs·Federica FarabegoliAna García Cabado
May 11, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yong MengBo-Zhong Mu
Apr 21, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yohei SakaguchiHitoshi Nohta

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