Detection of oligomeric forms of alpha-synuclein protein in human plasma as a potential biomarker for Parkinson's disease

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Omar M A El-AgnafDavid Allsop

Abstract

To date there is no accepted clinical diagnostic test for Parkinson's disease (PD) based on biochemical analysis of blood or cerebrospinal fluid (CSF). alpha-Synuclein (alpha-syn) protein has been linked to the pathogenesis of PD with the discovery of mutations in the gene encoding alpha-syn in familial cases with early-onset PD. Lewy bodies and Lewy neurites, which constitute the main pathological features in the brains of patients with sporadic PD and dementia with Lewy bodies, are formed by the conversion of soluble monomers of alpha-syn into insoluble aggregates. We recently reported the presence of alpha-syn in normal human blood plasma and in postmortem CSF. Here, we investigated whether alpha-syn can be used as a biomarker for PD. We have developed a novel ELISA method that detects only oligomeric "soluble aggregates" of alpha-syn. Using this ELISA, we report the presence of significantly elevated (P=0.002) levels of oligomeric forms of alpha-syn in plasma samples obtained from 34 PD patients compared with 27 controls; 52% (95% confidence intervals 0.353-0.687) of the PD patients displayed signals >0.5 OD with our ELISA assay in comparison to only 14.8% (95% confidence intervals 0.014-0.281) for the control cases. An ana...Continue Reading

References

Jun 1, 1988·Journal of Neurology, Neurosurgery, and Psychiatry·W R Gibb, A J Lees
May 23, 1994·FEBS Letters·R JakesM Goedert
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M G SpillantiniM Goedert
Oct 15, 1998·The New England Journal of Medicine·A E Lang, A M Lozano
Jan 29, 1999·BMJ : British Medical Journal·A H Schapira
May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·J BieschkeH Kretzschmar
Jul 13, 2000·Biochemical and Biophysical Research Communications·O M El-AgnafB M Austen
Jul 19, 2002·Nature·Hilal A LashuelPeter T Lansbury
Nov 26, 2002·Journal of Alzheimer's Disease : JAD·Qiao-Xin LiJanetta G Culvenor
Jul 25, 2003·Lancet Neurology·Omar M A El-AgnafDavid Allsop
Oct 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Omar M A El-AgnafDavid Allsop
Nov 1, 2003·Science·A B SingletonK Gwinn-Hardy
Feb 3, 2004·Annals of Neurology·Juan J ZarranzJusto G de Yebenes
Jun 8, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Omar M A El-AgnafDavid Allsop
Jun 9, 2004·Protein and Peptide Letters·Francis L MartinOmar M A El-Agnaf
Sep 29, 2004·Lancet·Marie-Christine Chartier-HarlinAlain Destée
Jun 15, 2005·Annual Review of Biochemistry·Mark R Cookson

❮ Previous
Next ❯

Citations

Jun 27, 2012·Journal of Neural Transmission·Sagar KansaraWeidong Le
Jan 19, 2013·Journal of Neural Transmission·Giovanni CaranciAnnamaria Confaloni
Jan 30, 2010·Biotechnology Letters·Kaori TsukakoshiKazunori Ikebukuro
Oct 22, 2008·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Kathleen A Maguire-Zeiss, Howard J Federoff
Oct 12, 2012·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Dawn BéraudKathleen A Maguire-Zeiss
Jul 2, 2008·Current Neurology and Neuroscience Reports·Tiffini Voss, Bernard Ravina
Sep 3, 2010·Current Neurology and Neuroscience Reports·John C MorganKapil D Sethi
Dec 25, 2007·Journal of Molecular Neuroscience : MN·Seung-Jae Lee
Jun 27, 2013·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Sarah K Kaufman, Marc I Diamond
Dec 27, 2008·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Ilan HalperinSilvia A Mandel
Jul 19, 2008·Lancet Neurology·Joshua A SonnenThomas J Montine
Sep 18, 2010·Lancet Neurology·Elodie AngotPatrik Brundin
Jun 16, 2012·Analytical Chemistry·Kaori TsukakoshiKazunori Ikebukuro
May 5, 2011·Biochemistry·Alice Y YamCleo M Salisbury
May 25, 2012·Biochemistry·Irina SurguchevaAndrei Surguchov
May 14, 2011·Cell Death and Differentiation·J A SteinerP Brundin
May 3, 2011·Nature Reviews. Drug Discovery·Wassilios G MeissnerErwan Bezard
Nov 4, 2010·Nature Reviews. Neurology·Seung-Jae LeeEliezer Masliah
Jul 20, 2010·Nature Reviews. Neurology·Penelope FouldsDavid Allsop
Nov 15, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Michael R SierksShalini Prasad
Aug 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Clemens R ScherzerMichael G Schlossmacher
Jan 15, 2010·The Journal of Biological Chemistry·He-Jin LeeSeung-Jae Lee
May 24, 2012·The Journal of Biological Chemistry·Kwang Soo KimSang Myun Park
Jan 15, 2009·The Journal of Biological Chemistry·Sharareh EmadiMichael R Sierks
Feb 17, 2010·Brain : a Journal of Neurology·Zhen HongJing Zhang
Mar 28, 2012·Brain : a Journal of Neurology·David S GoldsteinYehonatan Sharabi
Sep 30, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Karin M DanzerPamela J McLean
Nov 26, 2009·The Journal of Pharmacology and Experimental Therapeutics·Preeti PutchaPamela J McLean
Oct 12, 2012·International Journal of Alzheimer's Disease·Kensaku KasugaTakeshi Ikeuchi
Jan 22, 2008·Dementia and Geriatric Cognitive Disorders·Dag AarslandClive Ballard
Sep 19, 2012·Neuroimmunomodulation·Marina A GrudenRobert D E Sewell
Jul 11, 2006·The Journal of Clinical Investigation·Joseph M SavittTed M Dawson
Jan 20, 2011·The Journal of Clinical Investigation·Christian HansenPatrik Brundin
Aug 28, 2012·Molecular Neurodegeneration·Karin M DanzerPamela J McLean

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alpha-Synuclein Aggregation

Alpha-synucleins are small proteins that are believed to restrict the mobility of synpatic vesicles and inhibit neurotransmitter release. Aggregation of these proteins have been linked to several types of neurodegenerative diseases including dementia with Lewy bodies and Parkinson's disease. Here is the latest research on α-synuclein aggregation.

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.

Alpha-Synuclein Aggregation (MDS)

Alpha-synucleins are small proteins that are believed to restrict the mobility of synpatic vesicles and inhibit neurotransmitter release. Aggregation of these proteins have been linked to several types of neurodegenerative diseases including dementia with Lewy bodies and Parkinson's disease. Here is the latest research on α-synuclein aggregation.