PMID: 15387946Sep 25, 2004Paper

Detection of P504S (alpha-methylacyl-CoA racemase) in prostatic adenocarcinomas

Zhonghua yi xue za zhi
Yu XiaoFeng Jin

Abstract

To evaluate the specificity and sensitivity of the monoclonal antibody P504S (AMACR) in detection of prostatic adenocarcinomas. 150 cases, including prostatic adenocarcinomas (n = 105), benign prostatic hyperplasia (BPH, n = 42) and atypical small acinar proliferation (ASAP, n = 3), were studied by immunohistiochemical analysis of P504S. The clinical data, HE, PSA and CK34betaE12 staining slides were reviewed in all of the cases. P504S was strongly and diffusely positive (> or = +++) in 97.1% cases of prostatic adenocarcinomas and focally positive (+) in 2 cases, regardless of Gleason score, age and serum PSA. However, P504S was also positive in high grade PIN that surrounded adenocarcinomas (n = 24) and weakly positive (+) in benign prostatic hyperplasia that surrounded adenocarcinomas (8 out of 82 cases). In 42 cases of BPH, 10 cases (23.8%) show (+) staining, and 1 case (2.4%) shows (++) staining. P504S was negative in entire 8 cases of basal cell hyperplasia (BCH) and in 3 cases of ASAP. P504S (AMACR), a recently established positive marker for prostatic adenocarcinomas, has good sensitivity (97.1%) and specificity (86.0%). It is crucial to correlate the P504S staining pattern with the findings of HE, PSA and CK34betaE12 (o...Continue Reading

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