Detection of p53 gene abnormality by sequence analysis of archival paraffin tissue. A comparison with fresh-frozen specimens

Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B
M NadjiAzorides R Morales

Abstract

This parallel study was designed to compare the sensitivity and specificity of detection of point mutations in fresh-frozen and formalin-fixed, paraffin-embedded breast cancer tissue. Sequence analysis of exon 5 of p53 gene was performed on polymerase chain reaction-amplified DNA from 25 infiltrating ductal carcinomas of the breast. Four tumor showed mutations with identical base substitutions in their respective codons of both frozen and paraffin-embedded specimens. We conclude that subtle genetic alterations can be detected in archival paraffin tissue with an accuracy comparable to that of fresh-frozen histologic samples.

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Citations

Nov 11, 1998·Cancer Genetics and Cytogenetics·C WongM S Piver
May 3, 2005·Journal of Cancer Research and Clinical Oncology·Wolfgang J KöstlerChristoph C Zielinski
Jun 22, 2000·International Journal of Cancer. Journal International Du Cancer·R KandelT Rohan
Mar 6, 2004·Breast Cancer Research and Treatment·Hong LaiShenghan Lai
Aug 24, 2001·Breast Cancer Research and Treatment·E JanschekR Jakesz
Feb 17, 1999·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·T SugaiC Itoh
Oct 9, 2003·Breast Cancer Research and Treatment·Hong LaiEdward Trapido

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