Detection of p53 gene mutations in oral squamous cell carcinomas of a black African population sample

Human Mutation
E J van RensburgE J Raubenheimer

Abstract

Mutations in the p53 gene have been reported in head and neck carcinomas. We determined the p53 mutation profile in 55 oral squamous cell carcinomas (OSCCs) from a black African population sample. DNA from all the patients were investigated using PCR amplification of the p53 gene (exons 5-9), followed by heteroduplex single-stranded conformational polymorphism (HEX-SSCP) analysis on the PCR products. Direct sequencing was performed on cases where mutations were identified. The results showed mutations in 13 of 55 (23.6%) tumours. Eleven of 13 (85%) were single base pair substitutions (9 transitions and 2 transversions), and 2 were deletions. Two novel mutations were identified: a large 63-base pair deletion, and a single base pair substitution. The mutations in our study occurred outside the head and neck tumour hot spot region (codons 238-248).

References

Mar 15, 1979·Nature·D P Lane, L V Crawford
Dec 2, 1992·International Journal of Cancer. Journal International Du Cancer·E SakaiN Tsuchida
Aug 21, 1992·Cell·B Vogelstein, K W Kinzler
Jun 6, 1991·Nature·A J LevineC A Finlay
Jun 1, 1990·Biochimica Et Biophysica Acta·A J Levine, J Momand
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M C HollsteinC C Harris
Jun 1, 1989·Journal of the American College of Cardiology·T A Sanborn
May 1, 1986·Molecular and Cellular Biology·P Lamb, L Crawford
Mar 1, 1988·Head & Neck Surgery·R J Cusumano, M S Persky
Mar 11, 1988·Nucleic Acids Research·H S Bilofsky, C Burks
Oct 6, 1985·Nature·J R JenkinsG A Currie
Dec 22, 1994·Nature·A ZieglerD E Brash
Sep 1, 1994·Archives of Otolaryngology--head & Neck Surgery·W M KochD Sidransky
Jul 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G P Zambetti, A J Levine
Oct 1, 1993·Archives of Otolaryngology--head & Neck Surgery·J K FieldJ L Gluckman
Apr 1, 1993·Cancer Genetics and Cytogenetics·D Malkin
Feb 1, 1996·Journal of Epidemiology and Community Health·Z F ZhangX Z Yang

❮ Previous
Next ❯

Citations

Aug 14, 1999·Arthritis and Rheumatism·F KullmannU Müller-Ladner
Oct 12, 2004·The Laryngoscope·George H YooMichael A Tainsky
May 3, 2007·The Laryngoscope·Anthony C Nichols, Neil Bhattacharyya

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.