Detection of p53 point mutations by single strand conformation polymorphism: analysis by capillary electrophoresis

Electrophoresis
D H AthaC D O'Connell

Abstract

We have analyzed five p53 single point mutations by single strand conformation polymorphism using capillary electrophoresis (CE-SSCP) and have compared these measurements to measurements obtained by slab gel electrophoresis (SG-SSCP). PCR primers were used for amplification of specific exons for mutation detection. 5' Primers were labeled with FAM (5-carboxyfluorescein) and 3' primers were labeled with JOE (2',7'-dimethoxy-4',5'-dichloro-6-carboxyfluorescein). CE-SSCP was performed using the Perkin Elmer ABI PRISM 310 Genetic Analyzer with GeneScan Software and the Beckman P/ACE 5510 CE equipped for laser-induced fluorescence detection. Although the shifts in migration times for the p53 mutations relative to the corresponding wild-type strands could be successfully detected by either SG or CE analysis, the individual electrophoresis run times were about tenfold faster and more automated with capillary electrophoresis. The CE-SSCP measurements were performed at temperatures ranging from 10 to 60 degrees C on a prototype instrument. For mutations measured at ambient temperature (25 degrees C), characteristic shifts in direction and magnitude were observed in the migration times of both strands of all mutations relative to the wil...Continue Reading

References

Jul 5, 1991·Science·M HollsteinC C Harris
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M OritaT Sekiya
Nov 24, 1993·Journal of Chromatography·A W KuypersE J Mensink
Nov 24, 1995·Journal of Chromatography. a·T A FelmleeJ P Landers
Jun 1, 1996·Genome Research·M InazukaK Hayashi
Jan 26, 1996·Journal of Chromatography. B, Biomedical Applications·A W KuypersE J Mensink
Oct 25, 1996·Science·E S Lander
Jan 26, 1996·Journal of Chromatography. a·H ArakawaA Tsuji

❮ Previous
Next ❯

Citations

Oct 24, 2001·Electrophoresis·R KaczanowskiK Kucharczyk
Dec 30, 2004·Electrophoresis·Piotr Kozlowski, Wlodzimierz J Krzyzosiak
Jun 29, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·J Ren
Jul 14, 2001·Nucleic Acids Research·P Kozlowski, W J Krzyzosiak
Sep 2, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Donald H AthaVytas Reipa
Oct 29, 2003·Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology·Catherine D O'ConnellDonald H Atha
Nov 6, 2004·Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology·Bulbin Sunar-ReederCatherine D O'Connell
Jan 24, 2004·Vojnosanitetski pregled. Military-medical and pharmaceutical review·Gordana Zunić
Aug 1, 2008·Expert Opinion on Medical Diagnostics·Alison H DewaldJames P Landers
Oct 13, 2006·Electrophoresis·Christa N Hestekin, Annelise E Barron
Feb 9, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Piotr KozlowskiWlodzimierz J Krzyzosiak
Sep 8, 2015·Analytica Chimica Acta·Leonardo G GagliardiCecilia B Castells
Feb 21, 1998·Genome Research·H WenzJ W Efcavitch
Aug 20, 2019·Magnetic Resonance Imaging·Woo Kyung MoonRuey-Feng Chang
Nov 2, 2007·Chemical Reviews·Karel Kleparník, Petr Bocek

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.