Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology

Scientific Reports
Chao ZhangYongqiang Jiang

Abstract

Bloodstream infections (BSIs) are often life-threatening, and rapid identification is critical. Here, we developed a TaqMan array card (TAC) assay to detect pathogens in BSI specimens. The TAC included 30 primer/probe pairs targeting 27 species and 3 controls. Reverse transcription and 0.1% blue dextran 2000 increased the TAC assay efficiency. The primer/probe pairs had a limit of detection of 100-102 CFU/mL and a specificity of 100%. For whole blood specimens, the TAC assay showed a sensitivity and specificity of 79.4% and 99.69%, respectively. For blood culture samples, the TAC assay showed a sensitivity and specificity of 100% and 99.67%, respectively. The TAC assay could be a promising method for early detection of bloodstream infection.

References

Jan 17, 2002·The New England Journal of Medicine·E RiversUNKNOWN Early Goal-Directed Therapy Collaborative Group
Mar 7, 2003·Applied and Environmental Microbiology·Nichole E BrinkmanStephen J Vesper
Jan 13, 2010·Clinical Microbiology Reviews·Nicasio ManciniMassimo Clementi
Apr 8, 2011·Journal of Clinical Microbiology·Maja KodaniBarry S Fields
Feb 4, 2012·Journal of Clinical Microbiology·Michael J McConnellJerónimo Pachón
Apr 27, 2012·Journal of Microbiology and Biotechnology·Jung-Whan ChonKun-Ho Seo
Jun 1, 2012·Critical Care : the Official Journal of the Critical Care Forum·Arash AfshariStephan Harbarth
Nov 24, 2012·Journal of Clinical Microbiology·Jie LiuEric R Houpt
Mar 14, 2013·Expert Opinion on Medical Diagnostics·Antigone Kotsaki, Evangelos J Giamarellos-Bourboulis
May 13, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shunsuke FurutaniHidenori Nagai
Aug 8, 2014·Journal of Microbiological Methods·Marre van den BrandMartine P Bos
Oct 7, 2014·The Canadian Journal of Infectious Diseases & Medical Microbiology = Journal Canadien Des Maladies Infectieuses Et De La Microbiologie Médicale·Jeffrey M PernicaRobert Slinger
Apr 14, 2015·Expert Review of Molecular Diagnostics·Solomon MwaigwisyaJustin O'Grady
Nov 13, 2015·Expert Review of Molecular Diagnostics·Grégory Dubourg, Didier Raoult
Apr 14, 2017·Journal of Clinical Microbiology·Clayton O OnyangoBarry Fields
Jul 22, 2017·The Journal of International Medical Research·Andrej Markota, Andreja Sinkovič

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Methods Mentioned

BETA
PCR
PCRs
nucleic acid extraction

Software Mentioned

SPSS
BLAST
Clustal X
Primer Express

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