Detection of perinuclear antineutrophil cytoplasmic antibodies and antinuclear antibodies in the diagnosis of canine inflammatory bowel disease

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Carolina ManchoFernando Rodríguez-Franco

Abstract

In recent years, serologic markers for diagnosis and classification of inflammatory bowel disease (IBD) have been used in human medicine. Perinuclear, antineutrophil, cytoplasmic antibodies (p-ANCA) are the most important of these markers. Because of their similar pattern of fluorescence, antinuclear antibodies (ANA) could cause misleading interpretations. The aim of the present study was to evaluate the use of an indirect fluorescent antibody test to detect p-ANCA in dogs with IBD, to compare the presence of p-ANCA in dogs with IBD with the presence of the same antibodies in other dogs, and to analyze the presence of ANAs in the p-ANCA-positive samples. Using a 110 dilution as a cutoff point, a sensitivity of 0.34 and a specificity of 0.86 was obtained when dogs with IBD were compared with the other groups as a whole, and specificity increased to 0.94 when dogs with IBD were compared with animals with other chronic gastrointestinal disorders. The lowest specificity value, 0.76, was obtained when the group of dogs with IBD was compared with that of dogs with different inflammatory and infectious disorders. Globally, 78 dogs were positive for p-ANCA when the cutoff was 110. Only 1 dog from these 78 animals was also seropositive ...Continue Reading

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Citations

May 29, 2015·Journal of the South African Veterinary Association·Marlies Böhm, Sandra Basson
Apr 7, 2018·Journal of Veterinary Internal Medicine·Cristiane C OtoniAlbert E Jergens
Nov 8, 2013·Polish Journal of Veterinary Sciences·M WdowiakA Kołodziejska-Sawerska
Oct 4, 2018·Toxicologic Pathology·Rachel E Cianciolo, J Charles Jennette
Apr 14, 2020·Journal of Veterinary Internal Medicine·Juan J EstruchMichael T Sueda
Aug 8, 2013·Acta Veterinaria Hungarica·Andrzej RychlikAnna Kołodziejska-Sawerska
Sep 18, 2015·Veterinary Sciences·Guillaume Cazalot, Sidonie N Lavergne
May 8, 2021·Journal of Veterinary Internal Medicine·Juan EstruchPhilip Bergman
Apr 14, 2011·The Veterinary Clinics of North America. Small Animal Practice·Nora Berghoff, Jörg M Steiner

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Methods Mentioned

BETA
biopsy
biopsies
scraping

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