Detection of PERV by polymerase chain reaction and its safety in bioartificial liver support system

World Journal of Gastroenterology : WJG
Hai-Hui WangYu-Ming Wang

Abstract

To establish a method detecting porcine endogenous retrovirus (PERV) in China experimental minipigs and to evaluate the safety of PERV in three individuals treated with bioartificial liver support systems based on porcine hepatocytes. Porcine hepatocytes were isolated with two-stage perfusion method, then cultured in the bioreactor, which is separated by a semipermeable membrane (0.2 microm) from the lumen through which the patients' blood plasma was circulated. After post-hemoperfusion, patients' blood was obtained for screening. Additionally, samples of medium collected from both intraluminal and extraluminal compartments of the laboratory bioreactor and culture supernate in vitro was analyzed. The presence of viral sequences was estimated by polymerase chain reaction (PCR) and reverse transcriptase-polymerase chain reaction (RT-PCR). Finally, the infection of virus in the supernate of common culture was ascertained by exposure to the fetal liver cells. PERV-specific gag sequences were found in the porcine hepatocytes using RT-PCR. and were detected in all samples from the intraluminal, extraluminal samples and culture supernate. However, culture supernatant from primary porcine hepatocytes (cleared of cellular debris) failed...Continue Reading

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Citations

Sep 22, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jinyang GuYitao Ding
May 16, 2014·Viruses·Magdalena C KimsaUrszula Mazurek
Apr 5, 2008·Current Opinion in Critical Care·Jason Phua, Kang Hoe Lee
Nov 23, 2010·Frontiers of Medicine·Yi-Tao Ding, Xiao-Lei Shi
Dec 17, 2010·World Journal of Gastrointestinal Surgery·Robert Afm Chamuleau
Aug 19, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Jan-Henning FrühaufAugustinus Bader
Apr 17, 2012·The International Journal of Artificial Organs·Xiao-Lei ShiYi-Tao Ding
Jul 14, 2010·Fundamental & Clinical Pharmacology·Michael MaringkaAugustinus Bader
Apr 6, 2019·Artificial Organs·Yimin Zhang, Lanjuan Li

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