Detection of peste des petits ruminants virus antigen using immunofiltration and antigen-competition ELISA methods

Veterinary Microbiology
G Dhinakar RajM S Shaila

Abstract

Peste des petits ruminants (PPR) is one of the most economically important diseases affecting sheep and goats in India. An immunofiltration-based test has been developed using either mono-specific serum/monoclonal antibodies (mAb) prepared against a recombinant truncated nucleocapsid protein of rinderpest virus (RPV) cross-reactive with PPR virus. This method consists of coating ocular swab eluate from suspected animals onto a nitrocellulose membrane housed in a plastic module, which is allowed to react with suitable dilutions of a mAb or a mono-specific polyclonal antibody. The antigen-antibody complex formed on the membrane is then detected by protein A-colloidal gold conjugate, which forms a pink colour. In the immunofiltration test, concordant results were obtained using either PPRV mAb or mono-specific serum. Another test, an antigen-competition ELISA which relies on the competition between plate-coated recombinant truncated 'N' protein of RPV and the PPRV 'N' protein present in ocular swab eluates (sample) for binding to the mono-specific antibody against N protein of RPV (in liquid phase) was developed. The cut-off value for this test was established using reverse transcription polymerase chain reaction (RT-PCR) positive...Continue Reading

References

May 1, 1995·Veterinary Microbiology·A DialloM Barbron
Aug 1, 1996·Veterinary Microbiology·Y P NandaW P Taylor
Apr 27, 2001·Small Ruminant Research : the Journal of the International Goat Association·G Dhinakar RajK Nachimuthu
Dec 18, 2001·Journal of Virological Methods·E Couacy-HymannA Diallo
Jul 24, 2002·Veterinary Microbiology·Pronab DharS K Bandyopadhyay
Mar 18, 2003·The Veterinary Record·G Dhinakar RajK S Palaniswami
Jul 10, 2004·Clinical and Diagnostic Laboratory Immunology·Kang-Seuk ChoiYi-Seok Joo

❮ Previous
Next ❯

Citations

Dec 4, 2008·Tropical Animal Health and Production·Paul B Rossiter, Najib Al Hammadi
Feb 2, 2010·Journal of Virological Methods·Olivier KwiatekGenevieve Libeau
Sep 17, 2010·The Journal of General Virology·Ashley C BanyardGenevieve Libeau
Nov 23, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Ozlem BüyüktanirNevzat Yurdusev
Jan 11, 2011·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Oktay GençNevzat Yurdusev
Sep 17, 2010·Expert Review of Molecular Diagnostics·Vinayagamurthy BalamuruganRaj Kumar Singh
Feb 16, 2013·Food and Environmental Virology·Steven M LipsonRonald E Gordon
Dec 21, 2011·Journal of Immunoassay & Immunochemistry·Ozlem BüyüktanırNevzat Yurdusev
Jan 31, 2020·Acta Veterinaria Scandinavica·Edson KinimiGerald Misinzo

❮ Previous
Next ❯