Abstract
Alternative therapy for Helicobacter pylori eradication from natural products is gaining much attention. This study sought to isolate and characterize the fraction responsible for the antibacterial activity in Goldcrest (GC) n-hexane extract. Thin-layer chromatography (TLC) of the extract was carried out on Silica gel plates to determine the presence of chemical compounds, which were separated and partially purified by column chromatography. The obtained fractions GCCL, GCF2, GCF3 and GCF4 were tested for anti-H. pylori activity using the broth microdilution method. Volatile compounds in the active fractions were identified by gas chromatography-mass spectrometry (GC-MS) analysis. MINITAB was used for statistical analysis at 95% confidence interval. The best antibacterial activity was exhibited by GCF3 (5 mg/mL), which was composed of many compounds with known antimicrobial and antioxidant properties. A total of 16 volatile compounds were identified from fractions GCF2, GCF3 and GCF4 into the following families; alcohol, ketone, aliphatic acid, benzene compound, hydrocarbon, furan and pyran derivatives. The demonstration of antibacterial activity by the column fractions of GC n-hexane extract may provide new lead molecules that...Continue Reading
References
May 16, 2000·Journal of Bacteriology·D KersulyteD E Berg
Oct 19, 2000·Antimicrobial Agents and Chemotherapy·D H KwonD Y Graham
Dec 18, 2002·Helicobacter·Hans-Christoph E ZschauschMarkus J Maeurer
Aug 13, 2004·Gut·F Mégraud
Jan 19, 2005·Tropical Medicine & International Health : TM & IH·R N NdipT Nkuo Akenji
Apr 9, 2005·Journal of Ethnopharmacology·Yang LiRen Xiang Tan
Mar 3, 2007·The Journal of Antimicrobial Chemotherapy·Vincenzo De FrancescoEnzo Ierardi
Dec 12, 2007·Journal of Separation Science·Constantine D Stalikas
Apr 4, 2008·Tropical Medicine & International Health : TM & IH·Roland N NdipLawrence T Weaver
Aug 25, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Igor JerkovićMirko Gugić
May 5, 2010·Journal of Medicinal Food·Mamdouh Abdulmaksoud AbdulrhmanAshraf Hassan Mohamed
May 19, 2010·Nucleic Acids Research·Wolfgang FischerRainer Haas
Jul 20, 2010·Diagnostic Microbiology and Infectious Disease·Lyudmila BoyanovaIvan Mitov
Sep 21, 2010·Archives of Medical Research·Christy E Manyi-LohRoland N Ndip
Jul 5, 2011·Archives of Medical Research·Collise NjumeRoland N Ndip
Feb 9, 2012·Malaria Research and Treatment·Denis ZofouVincent P K Titanji
Citations
Apr 30, 2014·Journal of Applied Microbiology·F KhanS van Vuuren
Sep 23, 2014·Archives of Medical Research·Fredrick Matongo, Uchechukwu U Nwodo
May 28, 2016·Journal of Enzyme Inhibition and Medicinal Chemistry·Nimet BaltasSevgi Kolayli
Jul 1, 2017·Gastroenterology Research and Practice·Daniel YordanovIvan Mitov
Jul 14, 2017·Frontiers in Pharmacology·Laura CornaraBruno Burlando
Oct 30, 2019·World Journal of Gastroenterology : WJG·Xiao-Bei SiLin-Yu Huo