Detection of plant viruses using a surface plasmon resonance via complexing with specific antibodies

Journal of Virological Methods
P M BoltovetsYu M Shirshov

Abstract

The use of instrumental systems based on the surface plasmon resonance (SPR) for rapid diagnosis of intact plant viruses (in particular, tobacco mosaic virus (TMV)) is considered. A new approach using detection of viral antigen and antibody (IgG) complexes formed during the preincubation step (instead of their consecutive application in classical approach) is discussed. A comparison between signal level registered from the mixture of virus and specific serum and that from the sample without virus (samples deposited onto the sensor surface treated with thiocyanate and protein A Staphylococcus aureus) allows unambiguous detection of viral particles in the material studied. The performance capabilities of the method are discussed and illustrated by quantitative detection of virus in the actual samples (cells homogenate) at high concentration.

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Citations

Aug 22, 2007·Analytical and Bioanalytical Chemistry·Jie XuDavid S Gottfried
Jan 19, 2013·Applied Microbiology and Biotechnology·Pooja AroraAshok Chaudhury
Oct 28, 2011·Environmental Science & Technology·Gregory MarusovMichael A Lynes
May 13, 2008·Applied and Environmental Microbiology·Cristina García-AljaroFrancesc Xavier Muñoz
Jan 11, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yan LiToshihiko Imato
Jul 7, 2014·Colloids and Surfaces. B, Biointerfaces·Zeinab HosseinidoustNathalie Tufenkji
Feb 19, 2013·International Journal of Food Microbiology·Betsy Jean YakesStacey L Degrasse
Feb 14, 2006·Biotechnology and Bioengineering·Hyoungwon BaacMichael L Shuler
Nov 22, 2005·Analytical Biochemistry·D Keith Roper, Shamit Nakra
Nov 20, 2012·The Analyst·Praskoviya M BoltovetsBoris A Snopok
Aug 17, 2016·Biosensors·Penmetcha K R Kumar
Mar 1, 2014·Scientific Reports·Hadi ShafieeUtkan Demirci
Oct 28, 2005·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Jun 22, 2011·Lab on a Chip·Gerald BirnbaumerPeter Ertl

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