Detection of plasmid-mediated colistin-resistant and carbapenem-resistant genes by multiplex PCR

MethodsX
Rujirat HatrongjitShigeyuki Hamada

Abstract

A multiplex PCR was described to simultaneously detect mcr-1 and frequently occurring carbapenem-resistant genes including blaKPC, blaNDM, blaIMP, and blaOXA-48-like in a single reaction. The PCR product sizes of these 4 carbapenem-resistant genes were 232 bp, 438 bp, 621 bp, and 798 bp for blaIMP, blaOXA-48-like, blaNDM, and blaKPC, respectively, whereas mcr-1 revealed 1126 bp of PCR product. This protocol accurately detected those resistant genes in agreement with the reference strains, 127 local carbapenem-resistant Enterobacteriaceae, 8 mcr-1 carrying Enterobacteriaceae, and 62 carbapenem-susceptible Enterobacteriaceae. This method will be useful for laboratory application and surveillance of carbapenem and/or colistin-resistant bacteria.

Citations

Aug 20, 2019·Open Forum Infectious Diseases·Zekun LiQiwen Yang
Dec 17, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Aunyarat KhanawapeeShigeyuki Hamada
Jan 29, 2021·Microorganisms·Peechanika ChopjittAnusak Kerdsin
Jun 22, 2021·Journal of Applied Microbiology·Tumisho Mmatumelo Seipei LeshabaJohn Osei Sekyere

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Methods Mentioned

BETA
PCR
electrophoresis

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