PMID: 7020240Nov 1, 1980Paper

Detection of platelet antibody using rosette technique with anti-IgG antibody-coated polyacrylamide gel: a preliminary report

Vox Sanguinis
S SalmassiY Matsui

Abstract

Paraformaldehyde-fixed platelets from normal donors were used for detection of antibody to platelet by in vitro sensitization (indirect method) utilizing rabbit anti-human IgG heavy chain specific antibody-coated polyacrylamide gels (Immunobeads). The sensitized platelets formed rosettes with Immunobeads and the positive rosette count was over 30%, while control showed less than 8% when normal sera were used. This method was also applicable for detecting antibody-sensitized platelets in vivo (direct method) in patients with autoimmune thrombocytopenia. This method is simple, rapid and reproducible for clinical use. Direct and indirect immunofluorescent antibody tests and a blocking test with anti-human serum also supported the results of Immunobead rosetting technique.

References

Jul 1, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·W Chao, M M Yokoyama
Jun 1, 1978·British Journal of Haematology·A E von dem BorneC P Engelfriet
Sep 1, 1978·Tissue Antigens·O J Bergh, B G Solheim
Jan 1, 1977·International Archives of Allergy and Applied Immunology·J A MaplesW Chao
Jan 1, 1973·Scandinavian Journal of Immunology·C J van BoxtelT E Feltkamp

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Citations

Dec 1, 1983·The American Journal of Medicine·T R Burns, A Saleem
Oct 1, 1987·American Journal of Reproductive Immunology and Microbiology : AJRIM·R J Lau

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