PMID: 6169002Jun 25, 1981Paper

Detection of poly A+ RNA in sea urchin eggs and embryos by quantitative in situ hybridization

Nucleic Acids Research
L M Angerer, R C Angerer

Abstract

We present an improved procedure for detecting poly A tracts in situ by hybridization of 3H poly U. Glutaraldehyde fixation achieves significantly higher retention of RNA and better morphologic preservation than does Carnoy's. A dramatic increase in signal to noise is obtained by prehybridization treatment of glutaraldehyde-fixed sections with proteinase K and acetic anhydride. Measurement of the increase in poly A concentration after fertilization by solution titration and by in situ hybridization are in excellent agreement indicating that in situ measurements yield accurate relative estimates of local RNA concentrations in sections. Examination of the grain density distribution in section of sea urchin eggs and cleaving embryos reveals no major cytoplasmic localization of poly A+ RNA, although nuclei show much less labelling and micromeres of 16-cell embryos have a small, but significant, reduction in poly A concentration.

References

Jun 25, 1979·Nucleic Acids Research·C Godard, K W Jones
Aug 1, 1978·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S HayashiG M Tener
Dec 15, 1975·International Journal of Cancer. Journal International Du Cancer·M H Moar, K W Jones
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M Brahic, A T Haase
Jun 1, 1976·Developmental Biology·C Darnbrough, P J Ford
Jul 5, 1975·Journal of Molecular Biology·L M Dubroff, M Nemer
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·F H Wilt
Mar 1, 1966·Experimental Cell Research·G L AdaG J Nossal
Sep 1, 1980·Developmental Biology·S G ErnstE H Davidson
Mar 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·P R GROSSW A MOYER

❮ Previous
Next ❯

Citations

Feb 1, 1991·Molecular Reproduction and Development·Z Y GongB P Brandhorst
Jul 15, 1987·Experientia·J D PenschowJ P Coghlan
Nov 1, 1983·Developmental Biology·D V DeLeonR C Angerer
Oct 1, 1983·Developmental Biology·W R JefferyR D Brodeur
Feb 1, 1984·Developmental Biology·L M AngererR A RafF
Aug 1, 1986·Developmental Biology·L M AngererR C Angerer
Jun 1, 1987·Developmental Biology·B R Hough-EvansE H Davidson
Sep 1, 1987·Developmental Biology·K I SwensonJ V Ruderman
Sep 1, 1988·Developmental Biology·B R Hough-EvansE H Davidson
Jun 1, 1988·Developmental Biology·S M LoboG Schatten
Feb 1, 1990·Developmental Biology·J G HeuerM Bothwell
Nov 19, 1991·Brain Research. Developmental Brain Research·M D Willcutts, M Morrison-Bogorad
Sep 29, 1995·Brain Research. Developmental Brain Research·C E BeattieR E Siegel
Jan 1, 1993·Trends in Neurosciences·P C Emson
Jun 1, 1989·Brain Research. Molecular Brain Research·D L BensonE G Jones
Jan 1, 1990·Progress in Neurobiology·P J Harrison, R C Pearson
Apr 1, 1991·Molecular and Cellular Endocrinology·G SalbertP Jego
Jul 1, 1997·Brain Research. Molecular Brain Research·A Soto-PriorC P Hamel
Oct 21, 2005·The Journal of Molecular Diagnostics : JMD·Elena Rodríguez-IñigoVicente Carreño
May 1, 1993·Zygote : the Biology of Gametes and Early Embryos·P Thillai-KoothanR J Aitken

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.