Abstract
Two-dimensional polyacrylamide gel electrophoresis combined with a non-enzymatic sample preparation technique is useful for analysing clinical tumour material. Using these techniques, we analysed the relationship between the histopathological findings in primary lung malignancies and the expression of a number of unidentified polypeptides that were detected in the molecular weight region 20-35 kDa. In this study 45 cases of primary lung cancer (PLC) (21 cases of adenocarcinoma, ten cases of squamous cell carcinoma, five cases of large-cell carcinoma, one case of adenosquamous cell carcinoma, five cases of small-cell carcinoma and three cases of carcinoid tumour) were examined. For reference, a human diploid fibroblast cell line (W138) and normal peripheral lymphocytes were used. Sixteen polypeptides were judged to be associated with histopathological features. These polypeptides seem to be valuable as differentiation markers. The simultaneous evaluation of these polypeptides and some other proliferation markers (e.g. PCNA, PCNA 'satellite', Numatin/protein B23 and lamin B) seems to clarify the characteristics of each case of PLC. Furthermore, it is possible to classify PLC based on the two-dimensional electrophoresis findings, ...Continue Reading
References
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D L GardE Lazarides
May 7, 1976·Analytical Biochemistry·M M Bradford
May 29, 1992·Biochemical and Biophysical Research Communications·J R StrahlerS M Hanash
Jun 1, 1990·Cell·E R Fearon, B Vogelstein
Apr 1, 1989·Japanese Journal of Cancer Research : Gann·T HiranoY Hayata
Nov 9, 1989·Nature·A M RosengardP S Steeg
Oct 15, 1989·International Journal of Cancer. Journal International Du Cancer·K PatelF S Walsh
Jun 1, 1986·Human Pathology·H NomoriS Watanabe
Nov 1, 1981·Analytical Biochemistry·J H Morrissey
Dec 15, 1982·Cancer·P A RadiceJ D Minna
Nov 25, 1980·Journal of Molecular Biology·D PaulinJ Perreau
Feb 1, 1994·The American Journal of Pathology·T HiranoG Auer
Mar 1, 1994·Electrophoresis·K OkuzawaG Auer
Mar 1, 1994·Electrophoresis·B BjellqvistJ E Celis
Oct 1, 1993·Electrophoresis·B FranzénG Auer
Citations
Oct 13, 1999·Gut·S F MossP R Holt
Feb 15, 2005·BMC Biotechnology·Sreelatha MelethHelen Kim
Apr 1, 2013·European Journal of Dentistry·Seda AydemirNevin Kartal
Apr 10, 1999·Electrophoresis·I FialkaL A Huber
Dec 2, 1999·FEBS Letters·Y ChumanS Linder
Mar 11, 2003·Technology in Cancer Research & Treatment·Hong Wang, S M Hanash
Apr 5, 2007·Mass Spectrometry Reviews·Ajit BhartiRavi Salgia
Oct 23, 2013·The American Journal of Surgical Pathology·Oluwole FadareJonathan L Hecht
Mar 21, 1998·Electrophoresis·L BiniP Tosi
May 12, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Matthew Meyerson, David Carbone
Jan 9, 2007·Respirology : Official Journal of the Asian Pacific Society of Respirology·Takefumi Kikuchi, David P Carbone
Oct 6, 2009·Biomarkers in Medicine·April Scott, Ravi Salgia
Sep 1, 2010·Oncology Letters·Peng ZhangDA-Lie Ma
Mar 1, 1997·Electrophoresis·B FranzénG Auer
May 19, 2005·International Journal of Cancer. Journal International Du Cancer·Takashi HiranoNorihiko Ikeda
Jun 15, 2005·Journal of Korean Medical Science·Jeong Seok HwaKy Hyun Chung
Dec 23, 1999·Electrophoresis·C SartoP Mocarelli
Sep 1, 2007·Proteomics. Clinical Applications·José Bermúdez-Crespo, José Luis López
Oct 26, 2000·Japanese Journal of Cancer Research : Gann·T HiranoH Kato
Jan 5, 2019·The American Journal of Surgical Pathology·Philip P C IpKa-Yu Tse
May 11, 1999·Drug Discovery Today·M J PageR Parekh
Jul 15, 2021·Pathology Oncology Research : POR·Sören WeidemannSarah Minner