Detection of porcine circovirus type 1 in commercial pig vaccines using polymerase chain reaction

The Veterinary Journal
Josefina QuintanaMariano Domingo

Abstract

The absence of extraneous viruses is a requirement in the quality control of vaccines for veterinary use in the European Pharmacopoeia. A polymerase chain reaction (PCR) assay for the detection of porcine circovirus type 1 (PCV1) and type 2 (PCV2) was evaluated in 18 commercial porcine vaccines. Since vaccine components may contain PCR enhancers or inhibitors, 13 of the studied vaccines (used as diluents) were subsequently spiked with different dilutions of PCV2 and tested by PCR. Although PCV2 DNA was not detected in any of the vaccines tested, PCV1 was detected in 2/18 vaccines (11%). Eleven out of 13 PCV2 spiked vaccines showed a positive PCR result. The lack of amplification observed in two spiked vaccines suggested that use of the PCR assay to detect PCV2 could depend on vaccine composition. The results of this exploratory study have demonstrated that PCR is a rapid and fairly sensitive method for the detection of porcine circoviruses as extraneous agents in vaccine products and can be used in the quality control of pig vaccines. The study has also indicated the need for optimising the sensitivity of PCR methods for PCV genome detection in vaccine products.

References

Jan 1, 1986·Archives of Virology·I TischerW Griem
Jun 25, 1994·The Veterinary Record·S Edwards, J J Sands
Oct 1, 1996·The Journal of Veterinary Medical Science·A KojimaR Harasawa
Dec 1, 1996·Biologicals : Journal of the International Association of Biological Standardization·T SasakiF Chino
Mar 10, 1998·The Journal of Veterinary Medical Science·S KatayamaY Shimizu
Sep 25, 1998·The Journal of General Virology·B M MeehanG M Allan
Feb 26, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·G M Allan, J A Ellis
May 8, 2000·Biologicals : Journal of the International Association of Biological Standardization·S A AudetJ Beeler
Oct 29, 2002·The Veterinary Quarterly·J Segalés, M Domingo
Apr 5, 2003·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·G M Rodríguez-ArriojaM Domingo
Oct 1, 2000·Avian Pathology : Journal of the W.V.P.A·D Todd

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Citations

Dec 3, 2008·American Journal of Veterinary Research·Steven KrakowkaJohn A Ellis
Dec 20, 2011·Virus Research·Nicolas RoseAndré Jestin
Sep 1, 2015·International Journal of Surgery·Joachim Denner, Nicolas J Mueller
Sep 17, 2013·Biotechnology Progress·Bin YangJohannes Blümel
Jan 10, 2012·Transboundary and Emerging Diseases·X J Meng
Apr 12, 2008·Acta Veterinaria Hungarica·Attila CságolaTamás Tuboly
Nov 26, 2010·Vaccine·Sally A BaylisAnnette Mankertz
Apr 21, 2017·Viruses·Joachim Denner, Annette Mankertz
Sep 7, 2017·Virology Journal·Joachim Denner
Oct 17, 2019·Frontiers in Veterinary Science·Shao-Lun ZhaiYun Xi
Sep 29, 2018·Xenotransplantation·Anbu K Karuppannan, Tanja Opriessnig

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