Detection of porcine parvovirus using nonradioactive nucleic acid hybridization

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
K OraveerakulT W Molitor

Abstract

Nonradioactive slot blot hybridization assays were established for the detection of porcine parvovirus (PPV), using either a digoxigenin-labeled DNA probe or a biotinylated RNA probe. All probes were prepared from a 3.3-kb Pst1-EcoR1 DNA fragment of the NADL8 isolate of PPV. The sensitivity and specificity of the probes in a slot blot system were evaluated in comparison with a 32P-radiolabeled RNA probe. Using an anti-digoxigenin alkaline phosphatase detection system, at least 1 ng of viral replicative form (RF) DNA, or the equivalent of 100 plaque forming units (PFU) of infectious virus, could be detected by the digoxigenin-labeled DNA probe. When the biotinylated RNA probe and a strepavidin-alkaline phosphatase detection system were employed, 0.1 ng of RF DNA, or the equivalent of 10 PFU of infectious virus, were detected, comparable to the sensitivity of the 32P-radiolabeled RNA probe. Hybridization was not observed with control DNA samples extracted from swine testicle cells, porcine kidney (PK-15) cells, uninfected mixed swine fetal tissue, or from an unrelated DNA virus (pseudorabies virus) infected PK-15 cells. Different isolates of PPV, namely NADL8, NADL2, KBSH, and Kresse, reacted on an equimolar basis in sensitivity ...Continue Reading

References

Feb 1, 1977·Australian Veterinary Journal·H S Joo, R H Johnson
Jan 1, 1976·Archives of Virology·H S JooR H Johnson
Sep 1, 1976·Australian Veterinary Journal·H S JooR H Johnson
Mar 1, 1989·Journal of Clinical Microbiology·J MoriB J Cohen
Jan 1, 1989·Archives of Virology·C B BruceD L Tyrrell
Oct 1, 1989·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·R F MeyerV N Vakharia
Sep 1, 1987·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·Sándor BelákT Linné
Oct 1, 1987·Veterinary Microbiology·C S ChoiR Gunther
Mar 1, 1988·Journal of Virological Methods·D A CunninghamR K Craig
Nov 1, 1988·Diagnostic Microbiology and Infectious Disease·N K ChatterjeeR Deibel
Nov 1, 1988·Diagnostic Microbiology and Infectious Disease·S M QadriM N Al-Ahdal
Jun 1, 1985·Journal of Virological Methods·T R MoenchD E Griffin
Feb 1, 1987·The Journal of Infectious Diseases·F Diaz-MitomaD L Tyrrell

Citations

Jan 1, 1993·Archives of Virology·K OraveerakulT W Molitor
May 1, 1994·Clinica Chimica Acta; International Journal of Clinical Chemistry·M MusianiM La Placa
May 1, 1991·Journal of Virological Methods·T W MolitorL R Ludemann
Jan 1, 1991·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·G E DuhamelW Lu
May 26, 2009·Molecular Biology Reports·Wei XieQihui Luo

Related Concepts

Metazoa
Medebiotin
Digoxigenin
DNA, Viral
Fetal Mummification
Genomic Hybridization
Parvoviridae
Disease due to Parvoviridae
Family suidae
Swine Diseases

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