PMID: 9431940Feb 12, 1998Paper

Detection of Porphyromonas gingivalis from saliva by PCR by using a simple sample-processing method

Journal of Clinical Microbiology
J MättöS Asikainen

Abstract

Simple sample-processing methods for PCR detection of Porphyromonas gingivalis, a major pathogen causing adult periodontitis, from saliva were studied. The ability to detect P. gingivalis from 118 salivary samples by PCR after boiling and Chelex 100 processing was compared with bacterial culture. P. gingivalis was detected three times more often by PCR than by culture. Chelex 100 processing of saliva proved to be effective in preventing PCR inhibition and was applied to determine the occurrence of P. gingivalis in saliva samples from 263 Finnish subjects between 5 and 80 years of age. The occurrence of P. gingivalis increased with age, and it was detected by PCR in the saliva of 5.0% of subjects between 5 and 10 years of age, 13.8% of subjects between 11 and 20 years of age, 13.4% of subjects between 21 and 30 years of age, and 63.3% of subjects between 31 and 80 years of age. The results indicate that P. gingivalis is a rare finding in saliva from periodontally healthy children and young adults but a frequent one in saliva from adult periodontitis patients.

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Citations

Jul 31, 2003·Journal of Periodontology·Başak DoğanSirkka Asikainen
Nov 25, 2003·Letters in Applied Microbiology·K KumagaiK Ito
May 19, 2005·Journal of Periodontology·Başak DoĝanSirkka Asikainen
Mar 15, 2012·Journal of Periodontology·Petteri KaresvuoEija Könönen
Dec 11, 2014·PeerJ·Mahmoud A El-MakliziEdwin Cruz-Rivera
Mar 11, 2000·Journal of Periodontology·A AmanoI Morisaki
Aug 17, 1999·Microbiology and Immunology·M SakamotoT Nakase
Feb 9, 2000·Journal of Applied Microbiology·Y HuJ C Meitzler
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Jan 18, 2005·FEMS Microbiology Reviews·Teresa OlczakCaroline Attardo Genco
May 19, 2005·Journal of Periodontology·Kiyoko TamuraTakashi Ooshima
May 7, 2009·Oral Microbiology and Immunology·W PapaioannouL Papagiannoulis
Mar 1, 2004·Journal of Applied Oral Science : Revista FOB·Carlos Ferreira Dos SantosAndrew Seth Greene
Nov 19, 2011·Clinical Oral Implants Research·M Quirynen, N Van Assche
Mar 15, 2011·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Wayne A ChittickJeffrey J Zimmerman
Mar 28, 2013·Geriatrics & Gerontology International·Rupali Agnihotri, Sumit Gaur
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Apr 18, 2014·Periodontology 2000·Eija Könönen, Hans-Peter Müller
Aug 9, 2020·Clinical and Experimental Dental Research·Nobuko NagaiSeikou Shintani
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Jun 24, 2004·Oral Microbiology and Immunology·M IdesawaK Ito
Jun 9, 2005·Clinical and Diagnostic Laboratory Immunology·Yeon-Joo ChoiWon-Jong Jang

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