PMID: 9435886Jan 1, 1997Paper

Detection of primary DNA damage in Chlamydomonas reinhardtii by means of modified microgel electrophoresis

Environmental and Molecular Mutagenesis
M ErbesA Wild

Abstract

The assessment of genotoxic potential in surface water requires test methods, among which are those that detect initial DNA damage in organisms of aquatic biocenosis. The microgel electrophoresis (MGE) "comet assay" was applied to a ubiquitous unicellular green alga (Chlamydomonas reinhardtii) to detect DNA damage caused by genotoxins. For this, the test protocol described by Singh NP et al. [Exp Cell Res 175: 184-191, 1988] was modified. Major modifications were the use of alkaline lysis buffer with ionic detergents and the reduction of preincubation and electrophoresis times. Short-time exposure of Chlamydomonas to the well-known genotoxicants 4-nitroquinoline-1-oxide (4-NQO), N-nitrosodimethylamine, and hydrogen peroxide led to dose-dependent DNA damage. Chlamydomonas responded very sensitively to treatment with increasing doses of 4-NQO. At a concentration of 25 nM, significant DNA damage was observed. At higher 4-NQO doses (> 100 nM), DNA damage was visible as complete DNA fragmentation into fine granules. N-Nitrosodimethylamine caused genotoxic effects at a concentration range from 0.014 to 0.14 mM without producing complete DNA fragmentation at the concentrations tested (highest dose, 140 mM). To evaluate the influence o...Continue Reading

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Citations

Apr 23, 2008·Cell Biology and Toxicology·Alok DhawanDevendra Parmar
Sep 19, 2014·Ecotoxicology·Ricardo Santiago MartinezMaría Elena Sáenz
Aug 14, 2010·Journal of Hazardous Materials·J HrenovićL Sekovanić

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