Detection of prions in skin punch biopsies of Creutzfeldt-Jakob disease patients.

Annals of Clinical and Translational Neurology
Angela MammanaPiero Parchi

Abstract

Prion real-time quaking-induced conversion (RT-QuIC) is an ultrasensitive assay detecting pathological aggregates of misfolded prion protein in biospecimens. We studied 71 punch biopsy skin samples of 35 patients with Creutzfeldt-Jakob disease (CJD), including five assessed in vitam. The results confirmed the high value of skin prion RT-QuIC for CJD diagnosis (89% sensitivity and 100% specificity) and support its use in clinical practice. Preliminary data based on a limited number of cases suggest that prion-seeding activity in the skin varies according to the prion strain, being higher in sporadic CJD subtypes linked to the V2 strain (VV2 and MV2K) than in typical CJDMM1.

References

Mar 19, 2014·Neurology·Vincenzo DonadioRocco Liguori
Nov 29, 2016·Annals of Neurology·Aaron FoutzJiri G Safar
Aug 26, 2017·Progress in Molecular Biology and Translational Science·Byron CaugheyAllison Kraus
Sep 2, 2017·Methods in Molecular Biology·Christina D OrrùByron Caughey
Sep 8, 2017·Scientific Reports·Alessia FranceschiniPiero Parchi
Nov 24, 2017·Science Translational Medicine·Christina D OrrúWen-Quan Zou
Oct 26, 2018·Journal of Neurology, Neurosurgery, and Psychiatry·Simone BaiardiPiero Parchi

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Citations

Feb 4, 2021·Voprosy virusologii·S L Kal'novT V Grebennikova
Feb 21, 2021·Lancet Neurology·Peter HermannInga Zerr
Apr 4, 2021·Pathogens·Thi-Thu-Trang Dong, Katsuya Satoh
May 1, 2021·Viruses·Mark P Figgie, Brian S Appleby
May 12, 2021·Nature Reviews. Neurology·Neil WatsonSuvankar Pal
May 19, 2021·Movement Disorders : Official Journal of the Movement Disorder Society·Angela MammanaPiero Parchi
Aug 18, 2021·Journal of the Neurological Sciences·Kosuke MatsuzonoShigeru Fujimoto

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Methods Mentioned

BETA
biopsy
ELISA
biopsies

Software Mentioned

Sigma Plot

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