Detection of protein analytes via nanoparticle-based bio bar code technology

Analytical Chemistry
Y Paul BaoUwe R Müller

Abstract

We describe a new format for the recently introduced bio bar code technology, which improves the dose response over 10,000-fold and thereby makes this technique analytically useful. Unlike other ultrasensitive protein detection methods, such as immuno-PCR or immuno-RCA, the bio bar code technique does not employ any enzymes to achieve detection limits in the attomolar range. By sandwiching a target between a magnetic bead and an amplifier nanoparticle, a multiplicity of bar code oligonucleotides are released for each captured target analyte. These surrogate bar code targets are then hybridized to microarrays and detected with silver-amplified gold nanoparticle probes. Using PSA detection as a model, we demonstrate a linear dose response over at least 4 orders of magnitude in both target concentration and concomitant signal and a 1000-fold improvement in detection limit compared to the best ELISA system.

References

Aug 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·B SchweitzerD C Ward
Mar 30, 2002·Nature Biotechnology·Barry SchweitzerStephen F Kingsmore
May 1, 2002·Nature Biotechnology·Simon FredrikssonUlf Landegren
Aug 7, 2003·Biochemical and Biophysical Research Communications·Michael AdlerChristof M Niemeyer
Sep 5, 2003·Journal of Immunoassay & Immunochemistry·Javed Siddiqui, Daniel G Remick
Feb 26, 2004·Journal of Immunological Methods·Ru-Qiang LiangKang-Cheng Ruan
Aug 17, 2004·Clinical Chemistry·Petri SavirantaBernhard H Geierstanger
Jan 22, 2005·Nucleic Acids Research·Y Paul BaoUwe R Müller
Feb 8, 2005·Proceedings of the National Academy of Sciences of the United States of America·Dimitra G GeorganopoulouChad A Mirkin
Mar 23, 2005·Trends in Biotechnology·Christof M NiemeyerRon Wacker
Sep 20, 2005·Nature Biotechnology·Gengfeng ZhengCharles M Lieber
Dec 15, 2005·Analytical Chemistry·C Shad ThaxtonChad A Mirkin

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Citations

Oct 12, 2007·Analytical Chemistry·Haley D HillChad A Mirkin
Jun 5, 2007·Nature Protocols·Jwa-Min NamJay T Groves
Jun 18, 2009·Chemical Communications : Chem Comm·Liyuan ChenSongqin Liu
Oct 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·C Shad ThaxtonChad A Mirkin
Mar 17, 2010·The Journal of Infectious Diseases·Shixing Tang, Indira Hewlett
Aug 13, 2008·Nanomedicine·Kiley A White, Nathaniel L Rosi
Mar 23, 2012·Sensors·Noelia DasilvaManuel Fuentes
Apr 1, 2009·Bioanalysis·Cangel Pui-Yee Chan
Jun 23, 2009·International Journal of Food Microbiology·Minghui YangAvraham Rasooly
May 27, 2009·Journal of Immunological Methods·Longyan ChenXian-En Zhang
Apr 10, 2009·Photodiagnosis and Photodynamic Therapy·R R AllisonC H Sibata
Apr 17, 2007·Annals of the New York Academy of Sciences·Michael G MaukHaim H Bau
Mar 27, 2007·Journal of Applied Microbiology·J R BranenA L Branen
Jun 4, 2015·Nanomedicine·Sona KrizkovaRene Kizek
Jun 28, 2011·Analytical Biochemistry·Xiaodong CaoSongqin Liu
Feb 19, 2010·Bioresource Technology·Xiaowen Lü, Shuxun Cui
Jul 8, 2009·Journal of Pharmaceutical and Biomedical Analysis·Hui JinGuo-mao Hu
Apr 4, 2018·Chemical Society Reviews·Zhaowei ChenXiaogang Qu
Jul 29, 2010·Protein Science : a Publication of the Protein Society·Antonietta ParracinoSteffen B Petersen
Jul 25, 2007·The Analyst·Hongquan ZhangX Chris Le
Aug 19, 2007·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Shixing TangIndira K Hewlett
Feb 26, 2008·Proteomics·Christopher J JohnsonJudit M Nagy
Nov 28, 2008·Electrophoresis·Ken-ichi OhnoAndreas Manz
Jan 12, 2007·Molecular BioSystems·Uwe R Müller
Oct 23, 2010·Expert Review of Molecular Diagnostics·Ken Cham Fai LeungSiu Kai Kong

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