PMID: 9189052May 1, 1997Paper

Detection of protein and mRNA of various components of the NADPH oxidase complex in an immortalized human chondrocyte line

British Journal of Rheumatology
P J MoultonJ T Hancock

Abstract

The immortalized human chondrocyte cell line C-20/A4 has the ability to produce superoxide constitutively at low levels of 5.4 x 10(-2) nmol/min/10(6) cells (S.E.M. = +/-0.5, n = 30) and at raised levels upon stimulation with ionomycin and phorbol 12-myristate 13-acetate. Priming and anti-priming effects of interleukin (IL)-1 beta and IL-4, respectively, are also demonstrated. Reverse transcriptase polymerase chain reaction (RT-PCR) amplification using oligonucleotide primers to components of the NADPH oxidase enzyme complex showed mRNA expression of p22-phox, p40-phox and p47-phox. Western blot analysis using polyclonal antisera indicated the presence of the p47-phox p67-phox polypeptide components. These results show that the C-20/A4 cells contain an NADPH oxidase-like complex, similar to that found in other cell types, which produces superoxide anions.

Citations

May 5, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·J A Browning, R J Wilkins
Sep 22, 2006·Antioxidants & Redox Signaling·Laurent GrangeFrancoise Morel
Jan 16, 2016·Biochimica Et Biophysica Acta·Panagiotis Lepetsos, Athanasios G Papavassiliou
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Apr 15, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Adam M Wegner, Dominik R Haudenschild
Jul 1, 2013·Cartilage·Susan Chubinskaya, Markus A Wimmer
Aug 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuao WuHang T Ta

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