Detection of protein-protein interactions in plants using the transrepressive activity of the EAR motif repression domain

The Plant Journal : for Cell and Molecular Biology
Kyoko Matsui, Masaru Ohme-Takagi

Abstract

The activities of many regulatory factors involve interactions with other proteins. We demonstrate here that the ERF-associated amphiphilic repression (EAR) motif repression domain (SRDX) can convert a transcriptional complex into a repressor via transrepression that is mediated by protein-protein interactions and show that transrepressive activity of SRDX can be used to detect such protein-protein interactions. When we fused a protein that interacts with a transcription factor with SRDX and co-expressed the product with the transcription factor in plant cells, the expression of genes that are targets of the transcription factor was suppressed by transrepression. We demonstrated the transrepressive activity of SRDX using FOS and JUN as a model system and used two MADS box plant proteins, PISTILLATA and APETALA3, which are known to form heterodimers. Furthermore, the transgenic plants that expressed TTG1, which is a WD40 protein and interacts with bHLH transcription factors, fused to SRDX exhibited a phenotype similar to ttg1 mutants by transrepression and the regions of TTG1 required for interaction to the bHLH protein were detected using our system. We also used this system to analyse a protein factor that might be incorporate...Continue Reading

References

Mar 22, 2001·The Journal of Biological Chemistry·V R Ramirez-Carrozzi, T K Kerppola
Jun 6, 2003·The Plant Journal : for Cell and Molecular Biology·Keiichiro HiratsuMasaru Ohme-Takagi
Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·Yingzhen YangThomas Jack
Jan 27, 2004·The Plant Cell·Shiv B TiwariTom J Guilfoyle
Jul 17, 2004·The Plant Journal : for Cell and Molecular Biology·Antoine BaudryLoïc Lepiniec
Oct 8, 2004·The Plant Journal : for Cell and Molecular Biology·Keren Bracha-DroriNir Ohad
Jul 19, 2005·The Plant Cell·Luz I A Calderon-VillalobosClaus Schwechheimer
Nov 22, 2005·Gene·Monika PapworthMichal Minczuk
Mar 29, 2006·Applied Microbiology and Biotechnology·Kouichi KurodaMitsuyoshi Ueda
Dec 7, 2006·Plant Biotechnology Journal·Kyoko MatsuiMasaru Ohme-Takagi
Aug 11, 2007·Bioscience, Biotechnology, and Biochemistry·Tsuyoshi NakagawaSumie Ishiguro
Feb 14, 2008·The Plant Journal : for Cell and Molecular Biology·Jan A Miernyk, Jay J Thelen
Feb 14, 2008·The Plant Journal : for Cell and Molecular Biology·Sylvie LalondeWolf B Frommer
Jun 6, 2008·The Plant Journal : for Cell and Molecular Biology·Kyoko MatsuiMasaru Ohme-Takagi
Nov 11, 2008·Developmental Biology·Antonio GonzalezAlan Lloyd
Dec 17, 2008·Nature Methods·Jianmin WuSampsa Hautaniemi

❮ Previous
Next ❯

Citations

Dec 15, 2011·Plant Molecular Biology·Magdy M MahfouzJian-Kang Zhu
Dec 1, 2014·BMC Plant Biology·Margherita DaminatoGiorgio Casadoro
Jan 29, 2013·Plant Science : an International Journal of Experimental Plant Biology·Joseph F Petolino, John P Davies
Jul 8, 2017·PloS One·Yordan S YordanovVictor B Busov

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.