Detection of Puumala virus in the tissue of infected naturally rodent hosts in the area of central Dinarides

Journal of Virological Methods
Edina Dervović, Mirsada Hukić

Abstract

Hantaviruses are the causative agents of haemorrhagic fever with renal syndrome (HFRS) in Euroasia and of hantavirus cardiopulmonary syndrome (HCPS) in the North, Central and South America. HFRS is endemic in the Balkan Peninsula, where sporadic cases or outbreaks have been reported. Puumala virus (PUUV) is the causative agent of nephropathia epidemica (NE), a mild form of HFRS. PUUV is carried by the bank voles (Myodes glareolus). In this study, we investigated viral RNA from 76 tissues samples (lung n=30, heart n=6, liver n=18 and kidney n=22) of infected naturall rodent hosts in the area of Central Dinarides caught in live traps. Puumala virus was extracted from 34,7% (16/46) rodents by nested reverse transcriptase polymerase chain reaction (RT-PCR) and real-time RT-PCR. Overall, 18 (21,4%) specimens of internal organs (kidney n=8, liver n=6, heart n=2 and lung n=2) were positive for PUUV. It was shown a high rodent infestation rate in a relatively low number of rodent and their organs, although mice were not caught during the time of high density population of host rodents.

References

Jan 1, 1986·Archives of Virology·R VerhagenG van der Groen
Sep 1, 1995·Kidney International·M Papadimitriou
Apr 1, 1996·Journal of Wildlife Diseases·B J WeiglerW T Sullivan
Oct 23, 1997·Journal of Medical Virology·M D BowenN Nowotny
Dec 3, 1999·Microbes and Infection·C A Hart, M Bennett
Aug 8, 2001·Microbes and Infection·D GarinM Bouloy
Nov 16, 2001·Microbes and Infection·D H KrügerA Lundkvist A
Mar 9, 2002·Nucleic Acids Research·Ian M MackayAndreas Nitsche
Aug 27, 2002·Emerging Infectious Diseases·Gert E OlssonR Thomas Palo
Dec 4, 2003·The Lancet Infectious Diseases·Jan ClementMarc Van Ranst
Jan 25, 2005·Journal of Virological Methods·Mohamed AitichouM Sofi Ibrahim
Jan 27, 2005·Viral Immunology·Piet MaesMarc Van Ranst
Apr 29, 2009·Microbes and Infection·Misa KorvaTatjana Avsic-Zupanc

❮ Previous
Next ❯

Citations

Sep 10, 2020·Frontiers in Cellular and Infection Microbiology·Danny NoackBarry H G Rockx
Dec 14, 2018·Clinical Kidney Journal·Enisa MesicSenaid Trnacevic

❮ Previous
Next ❯

Related Concepts

Related Feeds

Argentine Hemorrhagic Fever

Argentine hemorrhagic fever (AHF) is an endemo-epidemic disease caused by junín virus (JUNV), a member of the arenaviridae family. Discover the latest research on AHF here.